Literature DB >> 1938902

A novel, highly efficient gene-cloning system for Micromonospora strains.

M Hasegawa1, T Dairi, T Ohta, E Hashimoto.   

Abstract

A highly efficient gene-cloning system for Micromonospora olivasterospora, a producer of the antibiotic fortimicin A (astromicin), suited to shotgun cloning has been developed. The system is supported by two new advancements accomplished in this study. One is the construction of novel plasmid vectors pMO116, pMO126, pMO133, pMO136, and pMO217, all consisting of replicons from newly found Micromonospora plasmids and selectable markers cloned from a neomycin-producing Micromonospora strain. The other advancement is the establishment of a new protocol for bacterial protoplasting in which some kinds of sugar alcohols are added in precultures. Such sugar alcohols were found to sensitize a wide taxonomical range of bacteria to lysozyme. The system is reproducible and reliable and has a high efficiency of more than 10(6) CFU/micrograms of DNA.

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Year:  1991        PMID： 1938902      PMCID： PMC209056          DOI： 10.1128/jb.173.21.7004-7011.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  19 in total

1.  Fortimicins A and B, new aminoglycoside antibiotics. I. Producing organism, fermentation and biological properties of fortimicins.

Authors:  T Nara; M Yamamoto; I Kawamoto; K Takayama; R Okachi; S Takasawa; T Sato; S Sato
Journal:  J Antibiot (Tokyo)       Date:  1977-07       Impact factor: 2.649

2.  A new antibiotic XK-62-2 (Sagamicin). I. Isolation, physicochemical and antibacterial properties.

Authors:  R Okachi; I Kawamoto; S Takasawa; M Yamamoto; S Sato
Journal:  J Antibiot (Tokyo)       Date:  1974-10       Impact factor: 2.649

3.  DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.

Authors:  C J Thompson; J M Ward; D A Hopwood
Journal:  Nature       Date:  1980-07-31       Impact factor: 49.962

4.  Cloning and characterization of the sisomicin-resistance gene from Micromonospora inyoensis.

Authors:  S L Goldberg; J G Romero; Y M Deo
Journal:  J Antibiot (Tokyo)       Date:  1990-08       Impact factor: 2.649

5.  The separation and differentiation of the gentamicin complex.

Authors:  H Maehr; C P Schaffner
Journal:  J Chromatogr       Date:  1967-10

6.  Isolation and characterization of plasmids from Micromonospora zionensis and Micromonospora rosaria.

Authors:  T Oshida; K Takeda; T Yamaguchi; S Ohshima; Y Ito
Journal:  Plasmid       Date:  1986-07       Impact factor: 3.466

7.  Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli.

Authors:  T Kieser
Journal:  Plasmid       Date:  1984-07       Impact factor: 3.466

8.  Transformation of Streptomyces erythraeus.

Authors:  H Yamamoto; K H Maurer; C R Hutchinson
Journal:  J Antibiot (Tokyo)       Date:  1986-09       Impact factor: 2.649

9.  pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.

Authors:  T Kieser; D A Hopwood; H M Wright; C J Thompson
Journal:  Mol Gen Genet       Date:  1982

10.  Efficient transformation of Amycolatopsis orientalis (Nocardia orientalis) protoplasts by Streptomyces plasmids.

Authors:  P Matsushima; M A McHenney; R H Baltz
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490
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  7 in total

1.  Self cloning in Micromonospora olivasterospora of fms genes for fortimicin A (astromicin) biosynthesis.

Authors:  T Dairi; T Ohta; E Hashimoto; M Hasegawa
Journal:  Mol Gen Genet       Date:  1992-03

2.  Development of a self-cloning system for Actinomadura verrucosospora and identification of polyketide synthase genes essential for production of the angucyclic antibiotic pradimicin.

Authors:  T Dairi; Y Hamano; T Furumai; T Oki
Journal:  Appl Environ Microbiol       Date:  1999-06       Impact factor: 4.792

3.  Vector systems allowing efficient autonomous or integrative gene cloning in Micromonospora sp. strain 40027.

Authors:  Xiaohua Li; Xiufen Zhou; Zixin Deng
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

4.  Cloning, expression, and characterization of the Micromonospora viridifaciens neuraminidase gene in Streptomyces lividans.

Authors:  K Sakurada; T Ohta; M Hasegawa
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

5.  Organization and nature of fortimicin A (astromicin) biosynthetic genes studied using a cosmid library of Micromonospora olivasterospora DNA.

Authors:  T Dairi; T Ohta; E Hashimoto; M Hasegawa
Journal:  Mol Gen Genet       Date:  1992-12

6.  N-formimidoyl fortimicin A synthase, a unique oxidase involved in fortimicin A biosynthesis: purification, characterization and gene cloning.

Authors:  T Dairi; K Yamaguchi; M Hasegawa
Journal:  Mol Gen Genet       Date:  1992-12

7.  Characterization of TnmH as an O-Methyltransferase Revealing Insights into Tiancimycin Biosynthesis and Enabling a Biocatalytic Strategy To Prepare Antibody-Tiancimycin Conjugates.

Authors:  Ajeeth Adhikari; Christiana N Teijaro; Xiaohui Yan; Chin-Yuan Chang; Chun Gui; Yu-Chen Liu; Ivana Crnovcic; Dong Yang; Thibault Annaval; Christoph Rader; Ben Shen
Journal:  J Med Chem       Date:  2020-07-24       Impact factor: 7.446
  7 in total

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