| Literature DB >> 3571169 |
P Matsushima, M A McHenney, R H Baltz.
Abstract
Conditions for efficient transformation of Amycolatopsis orientalis (Nocardia orientalis) protoplasts by Streptomyces plasmid cloning vectors were identified. Three streptomycete plasmid origins of replication function in A. orientalis, as do the apramycin resistance gene from Escherichia coli, the thiostrepton resistance gene from Streptomyces azureus, and the tyrosinase gene from Streptomyces antibioticus. A. orientalis appears to express some restriction and modification, because highest transformation frequencies (10(6)/micrograms of DNA) were obtained when plasmid pIJ702 was modified by passage in A. orientalis.Entities:
Mesh:
Year: 1987 PMID: 3571169 PMCID: PMC212162 DOI: 10.1128/jb.169.5.2298-2300.1987
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490