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Literature DB >> 163342

Characterization of human papovavirus BK DNA.

P M Howley, M F Mullarkey, K K Takemoto, M A Martin.

Abstract

The DNA of the BK virus (BKV) human papovavirus was found to be heterogeneous, consisting of at least four discrete species of DNA. Only the largest of these four species, BKV DNA (i), which has a molecular weight calculated to be 96% that of simian virus 40 (SV40) DNA, was infectious. Homogeneous preparations of BKV DNA were obtained, however, from virions purified after low multiplicity infections of human embryonic kidney cells. BKV DNA (i) was shown to contain a single R-Eco RI and four R-Hind cleavage sites. The R-Eco RI site was localized in the largest R-Hind cleavage fragment. Radiolabeled BKV DNA reassociated slightly faster than SV40 DNA; 20 to 30% polynucleotide sequence homology was demonstrated between the genomes of BKV and SV40 when the reaction was monitored by chromatography on hydroxyapatite.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1975 PMID： 163342 PMCID： PMC354432

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

29 in total

1. Characterization of "heavy" and "light" SV40-like particles from a patient with PML.

Authors: M A Martin; L D Gelb; C Garon; K K Takemoto; T N Lee; G H Sack; D Nathans
Journal: Virology Date: 1974-05 Impact factor: 3.616

2. Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments.

Authors: J E Osborn; S M Robertson; B L Padgett; G M ZuRhein; D L Walker; B Weisblum
Journal: J Virol Date: 1974-03 Impact factor: 5.103

3. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy.

Authors: B L Padgett; D L Walker; G M ZuRhein; R J Eckroade; B H Dessel
Journal: Lancet Date: 1971-06-19 Impact factor: 79.321

4. Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors: B Hirt
Journal: J Mol Biol Date: 1967-06-14 Impact factor: 5.469

5. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome.

Authors: P A Sharp; U Pettersson; J Sambrook
Journal: J Mol Biol Date: 1974-07-15 Impact factor: 5.469

Characterization of human papovavirus BK DNA.

1. Characterization of "heavy" and "light" SV40-like particles from a patient with PML.

2. Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments.

3. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy.

4. Selective extraction of polyoma DNA from infected mouse cell cultures.

5. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome.

6. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.

7. Kinetics of renaturation of DNA.

8. Prevalence in England of antibody to human polyomavirus (B.k.).

9. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.

10. Comparison of two human papovaviruses with simian virus 40 by structural protein and antigenic analysis.

1. Restriction endonuclease cleavage map of the DNA of JC virus.

2. Infectivity of the DNA from four isolates of JC virus.

3. Viable deletion mutant of human papovavirus BK that induces insulinomas in hamsters.

4. Biological activity of polyoma viral DNA in mice and hamsters.

5. Characterization of the genome of the murine papovavirus K.

6. Transformation of human embryonic kidney cells by human papovarirus BK.

7. Physical mapping of BK virus DNA with SacI, MboII, and AluI restriction endonucleases.

8. Nucleotide sequence of the BK virus DNA segment encoding small t antigen.

9. Uniform representation of the human papovavirus BK genome in transformed hamster cells.

10. Electron microscope study of the base sequence homology between simian virus 40 and human papovavirus BK.