Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Comparison of two human papovaviruses with simian virus 40 by structural protein and antigenic analysis.

Literature DB >> 4363247

Comparison of two human papovaviruses with simian virus 40 by structural protein and antigenic analysis.

M F Mullarkey, J F Hruska, K K Takemoto.

Abstract

The proteins of simian virus 40 (SV40) and two human papovaviruses, the hemagglutinating BK virus and the non-hemagglutinating DAR virus, were analyzed and compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The virions of SV40 and DAR contain seven proteins. By molecular weight analysis the constituent proteins of SV40 and DAR are identical. Approximately 84% of the viral protein has a molceular weight of 45,000. The major protein of BK virus is 3,000 to 5,000 daltons lighter than the major proteins of SV40 and DAR viruses. The five most rapidly migrating proteins of BK virus are indistinguishable by molecular weight analysis from the corresponding proteins of SV40 and DAR viruses. Radial immunodiffusion and immunoelectrophoresis of whole virus gave lines of identity between SV40 and DAR when reacted with SV40 antibody. SV40 antiserum tested against BK virus and BK antiserum tested against SV40 virus showed no reactivity by complement fixation, immunodiffusion, or immunoelectrophoresis.

Entities: Chemical Species

Mesh：

Substances：

Year: 1974 PMID： 4363247 PMCID： PMC355409

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

14 in total

Comparison of two human papovaviruses with simian virus 40 by structural protein and antigenic analysis.

1. The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy.

2. Isolation of virus related to SV40 from patients with progressive multifocal leukoencephalopathy.

3. Capsid proteins of Simian virus 40.

4. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy.

5. New human papovavirus (B.K.) isolated from urine after renal transplantation.

6. Structure of simian virus 40. I. Purification and physical characterization of the virus particle.

7. Hamster ascitic fluids containing complement-fixing antibody against virus-induced tumor antigens.

8. Mutants of simian virus 40 differing in plaque size, oncogenicity, and heat sensitivity.

9. Structural proteins of simian virus 40: phosphoproteins.

10. Structural polypeptides of simian virus 40.

1. Transformation of human embryonic kidney cells by human papovarirus BK.

2. Nucleotide sequence of the BK virus DNA segment encoding small t antigen.

3. Malignant transformation of hamster kidney cells by BK virus.

4. Virion polypeptide composition of the human papovavirus BK: comparison with simian virus 40 and polyoma virus.

5. Characterization of human papovavirus BK DNA.

6. Production of lymphokine-like factors (cytokines) by simian virus 40-infected and simian virus 40-transformed cells.

7. Structural polypeptides of rabbit, bovine, and human papillomaviruses.

8. Radioisotopic labeling of human papovavirus (BK) by iodination and reductive alkylation.

9. Differences between BK virus and simian virus 40 structural proteins analysed by isoelectric focusing.

10. Transcription of BK virus DNA by Escherichia coli RNA polymerase: size and sequence analysis of RNA.