Literature DB >> 16151152

S434F in NrdE generates the thermosensitive phenotype of corynebacterium ammoniagenes CH31 and enhances thermolability by increasing the surface hydrophobicity of the NrdE(Ts) protein.

Hesham M Elhariry1, Jochen Meens, Matthias Stehr, Georg Auling.   

Abstract

The thermosensitive phenotype of strain CH31, a derivative of Corynebacterium ammoniagenes ATCC 6872, was allocated by cloning, sequencing, and genetic complementation to a single C-->T exchange in the nrdE (nucleotide reduction) gene at nucleotide 1301. Protein modeling indicates the impaired surface hydrophobicity of NrdE(Ts) due to the S434F transition.

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Year:  2005        PMID： 16151152      PMCID： PMC1214679          DOI： 10.1128/AEM.71.9.5582-5586.2005

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  30 in total

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Authors:  A Willing; H Follmann; G Auling
Journal:  Eur J Biochem       Date:  1988-01-04

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Journal:  Chem Biol       Date:  1995-12

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Authors:  A A Pakula; R T Sauer
Journal:  Nature       Date:  1990-03-22       Impact factor: 49.962

5.  A divalent metal site in the small subunit of the manganese-dependent ribonucleotide reductase of Corynebacterium ammoniagenes.

Authors:  U Griepenburg; K Blasczyk; R Kappl; J Hüttermann; G Auling
Journal:  Biochemistry       Date:  1998-06-02       Impact factor: 3.162

6.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  From RNA to DNA, why so many ribonucleotide reductases?

Authors:  P Reichard
Journal:  Science       Date:  1993-06-18       Impact factor: 47.728

9.  Structural studies of mutants of the lysozyme of bacteriophage T4. The temperature-sensitive mutant protein Thr157----Ile.

Authors:  M G Grütter; T M Gray; L H Weaver; T A Wilson; B W Matthews
Journal:  J Mol Biol       Date:  1987-09-20       Impact factor: 5.469

10.  Parameters of unbalanced growth and reversible inhibition of deoxyribnucleic acid synthesis in Brevibacterium ammoniagenes ATCC 6872 induced by depletion of Mn2+. Inhibitor studies on the reversibility of deoxyribonucleic acid synthesis.

Authors:  G Auling; M Thaler; H Diekmann
Journal:  Arch Microbiol       Date:  1980-09       Impact factor: 2.552
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