PURPOSE: Recent studies have proposed that mucositis development is the same throughout the gastrointestinal tract (GIT), as it is formed from one structure embryologically. Radiation-induced oral mucositis studies have outlined the key involvement of nuclear factor kappaB (NFkappaB) and cyclooxygenase-2 (Cox-2) in its pathobiology. The purpose of this study was therefore to investigate the expression of NFkappaB and Cox-2 in the irradiated colorectum and to correlate these with the associated histopathologic changes. METHODS AND MATERIALS: Colorectal tissues from 28 colorectal cancer patients treated with preoperative radiotherapy were analyzed for histopathologic changes using a variety of tissue staining methods. The expression of NFkappaB and Cox-2 in these tissues was investigated using immunohistochemistry. Changes in expression of these proteins were then correlated with the histopathologic changes. RESULTS: Radiation therapy caused injury to the normal colorectal tissue surrounding tumor site, particularly around the blood vessels. These changes were reflected in changes in NFkappaB and Cox-2 expression. CONCLUSIONS: We conclude that different regions of the GIT, the colorectum, and oral cavity have similar underlying mechanisms of radiation-induced mucositis. Understanding these mechanisms will allow new approaches to be developed to specifically target steps in the evolution of alimentary mucositis.
PURPOSE: Recent studies have proposed that mucositis development is the same throughout the gastrointestinal tract (GIT), as it is formed from one structure embryologically. Radiation-induced oral mucositis studies have outlined the key involvement of nuclear factor kappaB (NFkappaB) and cyclooxygenase-2 (Cox-2) in its pathobiology. The purpose of this study was therefore to investigate the expression of NFkappaB and Cox-2 in the irradiated colorectum and to correlate these with the associated histopathologic changes. METHODS AND MATERIALS: Colorectal tissues from 28 colorectal cancerpatients treated with preoperative radiotherapy were analyzed for histopathologic changes using a variety of tissue staining methods. The expression of NFkappaB and Cox-2 in these tissues was investigated using immunohistochemistry. Changes in expression of these proteins were then correlated with the histopathologic changes. RESULTS: Radiation therapy caused injury to the normal colorectal tissue surrounding tumor site, particularly around the blood vessels. These changes were reflected in changes in NFkappaB and Cox-2 expression. CONCLUSIONS: We conclude that different regions of the GIT, the colorectum, and oral cavity have similar underlying mechanisms of radiation-induced mucositis. Understanding these mechanisms will allow new approaches to be developed to specifically target steps in the evolution of alimentary mucositis.
Authors: Romany L Stansborough; Emma H Bateman; Noor Al-Dasooqi; Joanne M Bowen; Dorothy M K Keefe; Ann S J Yeoh; Richard M Logan; Eric E K Yeoh; Andrea M Stringer; Rachel J Gibson Journal: Support Care Cancer Date: 2017-02-07 Impact factor: 3.603
Authors: Romany L Stansborough; Noor Al-Dasooqi; Emma H Bateman; Joanne M Bowen; Dorothy M K Keefe; Richard M Logan; Ann S J Yeoh; Eric E K Yeoh; Andrea M Stringer; Rachel J Gibson Journal: Support Care Cancer Date: 2018-05-12 Impact factor: 3.603
Authors: Zhi Yi Ong; Rachel J Gibson; Joanne M Bowen; Andrea M Stringer; Jocelyn M Darby; Richard M Logan; Ann Sj Yeoh; Dorothy M Keefe Journal: Radiat Oncol Date: 2010-03-16 Impact factor: 3.481
Authors: Noor Al-Dasooqi; Joanne Bowen; Colin Bennett; John Finnie; Dorothy Keefe; Rachel Gibson Journal: Support Care Cancer Date: 2016-09-20 Impact factor: 3.603