Bonnie Williams1, Eleni Tsangari2, Romany Stansborough2, Victor Marino3, Melissa Cantley2, Anak Dharmapatni2, Rachel Gibson2,4, Egon Perilli5, Tania Crotti2. 1. Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia. bonnie.williams@adelaide.edu.au. 2. Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia. 3. School of Dentistry, The University of Adelaide, Adelaide, SA, Australia. 4. Division of Health Sciences, University of South Australia, Adelaide, SA, Australia. 5. School of Computer Science, Engineering and Mathematics, Flinders University, Adelaide, SA, Australia.
Abstract
OBJECTIVE: To investigate the effect of caffeic acid phenethyl ester (CAPE) on local and systemic inflammation and bone loss in collagen antibody-induced arthritis (CAIA) mice. METHODS: Four groups of mice (n = 8 per group) were allocated; control, CAPE (1 mg/kg), CAIA and CAIA + CAPE (1 mg/kg). Local inflammation and bone loss were evaluated using clinical paw scores, in vivo micro-computed tomography (micro-CT), histological assessment and tartrate-resistant acid phosphatase (TRAP) staining. Serum levels of C-reactive protein (CRP) and C-terminal telopeptide (CTX-1) were measured by ELISA. Jejunum and colon sections were evaluated histopathologically for damage and toxicity. RESULTS: Greater paw scores and percentage change in paw volume were observed in CAIA + CAPE compared to the control groups (p < 0.05). Bone volume over time remained unchanged (p = 0.94) and the number of multinucleated TRAP-positive cells was greatest in CAIA + CAPE mice (p < 0.05). CRP and CTX-1 levels did not differ between groups. CAIA + CAPE mice exhibited lower colon toxicity scores and a reduced percentage of cavitated goblet cells in the colon crypts compared with CAIA mice (p = 0.026 and p = 0.003, respectively). Histopathology in the jejunum was not altered. CONCLUSION: CAPE did not reduce paw inflammation or bone loss in CAIA mice. CAPE reduced histopathological changes in the colon of CAIA mice.
OBJECTIVE: To investigate the effect of caffeic acid phenethyl ester (CAPE) on local and systemic inflammation and bone loss in collagen antibody-induced arthritis (CAIA) mice. METHODS: Four groups of mice (n = 8 per group) were allocated; control, CAPE (1 mg/kg), CAIA and CAIA + CAPE (1 mg/kg). Local inflammation and bone loss were evaluated using clinical paw scores, in vivo micro-computed tomography (micro-CT), histological assessment and tartrate-resistant acid phosphatase (TRAP) staining. Serum levels of C-reactive protein (CRP) and C-terminal telopeptide (CTX-1) were measured by ELISA. Jejunum and colon sections were evaluated histopathologically for damage and toxicity. RESULTS: Greater paw scores and percentage change in paw volume were observed in CAIA + CAPE compared to the control groups (p < 0.05). Bone volume over time remained unchanged (p = 0.94) and the number of multinucleated TRAP-positive cells was greatest in CAIA + CAPEmice (p < 0.05). CRP and CTX-1 levels did not differ between groups. CAIA + CAPEmice exhibited lower colon toxicity scores and a reduced percentage of cavitated goblet cells in the colon crypts compared with CAIAmice (p = 0.026 and p = 0.003, respectively). Histopathology in the jejunum was not altered. CONCLUSION:CAPE did not reduce paw inflammation or bone loss in CAIAmice. CAPE reduced histopathological changes in the colon of CAIAmice.
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