Literature DB >> 16030206

Novel intracellular 3-hydroxybutyrate-oligomer hydrolase in Wautersia eutropha H16.

Teruyuki Kobayashi1, Keiichi Uchino, Tomoko Abe, Yuya Yamazaki, Terumi Saito.   

Abstract

Wautersia eutropha H16 (formerly Ralstonia eutropha) mobilizes intracellularly accumulated poly(3-hydroxybutyrate) (PHB) with intracellular poly(3-hydroxybutyrate) depolymerases. In this study, a novel intracellular 3-hydroxybutyrate-oligomer hydrolase (PhaZc) gene was cloned and overexpressed in Escherichia coli. Then PhaZc was purified and characterized. Immunoblot analysis with polyclonal antiserum against PhaZc revealed that most PhaZc is present in the cytosolic fraction and a small amount is present in the poly(3-hydroxybutyrate) inclusion bodies of W. eutropha. PhaZc degraded various 3-hydroxybutyrate oligomers at a high specific activity and artificial amorphous poly(3-hydroxybutyrate) at a lower specific activity. Native PHB granules and semicrystalline PHB were not degraded by PhaZc. A PhaZ deletion mutation enhanced the deposition of PHB in the logarithmic phase in nutrient-rich medium. PhaZc differs from the hydrolases of W. eutropha previously reported and is a novel type of intracellular 3-hydroxybutyrate-oligomer hydrolase, and it participates in the mobilization of PHB along with other hydrolases.

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Year:  2005        PMID： 16030206      PMCID： PMC1196019          DOI： 10.1128/JB.187.15.5129-5135.2005

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  27 in total

1.  Genome sequence of the plant pathogen Ralstonia solanacearum.

Authors:  M Salanoubat; S Genin; F Artiguenave; J Gouzy; S Mangenot; M Arlat; A Billault; P Brottier; J C Camus; L Cattolico; M Chandler; N Choisne; C Claudel-Renard; S Cunnac; N Demange; C Gaspin; M Lavie; A Moisan; C Robert; W Saurin; T Schiex; P Siguier; P Thébault; M Whalen; P Wincker; M Levy; J Weissenbach; C A Boucher
Journal:  Nature       Date:  2002-01-31       Impact factor: 49.962

2.  Purification of an extracellular D-(-)-3-hydroxybutyrate oligomer hydrolase from Pseudomonas sp. strain A1 and cloning and sequencing of its gene.

Authors:  K Zhang; M Shiraki; T Saito
Journal:  J Bacteriol       Date:  1997-01       Impact factor: 3.490

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  beta-Hydroxybutyric dehydrogenase and dimer hydrolase of Pseudomonas lemoignei.

Authors:  F P Delafield; K E Cooksey; M Doudoroff
Journal:  J Biol Chem       Date:  1965-10       Impact factor: 5.157

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC).

Authors:  O P Peoples; A J Sinskey
Journal:  J Biol Chem       Date:  1989-09-15       Impact factor: 5.157

7.  Catalytic triad of intracellular poly(3-hydroxybutyrate) depolymerase (PhaZ1) in Ralstonia eutropha H16.

Authors:  Teruyuki Kobayashi; Terumi Saito
Journal:  J Biosci Bioeng       Date:  2003       Impact factor: 2.894

8.  Cloning and sequencing of an intracellular D(-)-3-hydroxybutyrate oligomer hydrolase from Acidovorax sp. strain SA1 and purification of the enzyme.

Authors:  Akinori Sugiyama; Mari Shiraki; Teruyuki Kobayashi; Gakushi Morikawa; Mizuho Yamamoto; Michiyo Yamaoka; Terumi Saito
Journal:  Curr Microbiol       Date:  2002-08       Impact factor: 2.188

9.  Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes.

Authors:  Gregory M York; Joachim Lupberger; Jiamin Tian; Adam G Lawrence; JoAnne Stubbe; Anthony J Sinskey
Journal:  J Bacteriol       Date:  2003-07       Impact factor: 3.490

10.  DEPOLYMERIZATION OF POLY-BETA-HYDROXYBUTYRATE BY INTRACELLULAR ENZYME SYSTEM.

Authors:  J M MERRICK; M DOUDOROFF
Journal:  J Bacteriol       Date:  1964-07       Impact factor: 3.490
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  22 in total

1.  Poly(3-hydroxybutyrate) (PHB) depolymerase PhaZa1 is involved in mobilization of accumulated PHB in Ralstonia eutropha H16.

Authors:  Keiichi Uchino; Terumi Saito; Dieter Jendrossek
Journal:  Appl Environ Microbiol       Date:  2007-12-21       Impact factor: 4.792

Review 2.  Polyhydroxyalkanoate granules are complex subcellular organelles (carbonosomes).

Authors:  Dieter Jendrossek
Journal:  J Bacteriol       Date:  2009-03-06       Impact factor: 3.490

3.  Comparative proteome analysis reveals four novel polyhydroxybutyrate (PHB) granule-associated proteins in Ralstonia eutropha H16.

Authors:  Anna Sznajder; Daniel Pfeiffer; Dieter Jendrossek
Journal:  Appl Environ Microbiol       Date:  2014-12-29       Impact factor: 4.792

Review 4.  Genome characteristics dictate poly-R-(3)-hydroxyalkanoate production in Cupriavidus necator H16.

Authors:  Gurusamy Kutralam-Muniasamy; Fermín Peréz-Guevara
Journal:  World J Microbiol Biotechnol       Date:  2018-05-24       Impact factor: 3.312

5.  Identification and characterization of a novel class of extracellular poly(3-hydroxybutyrate) depolymerase from Bacillus sp. strain NRRL B-14911.

Authors:  Wan-Ting Ma; Ju-Hui Lin; Hui-Ju Chen; Syuan-Yi Chen; Gwo-Chyuan Shaw
Journal:  Appl Environ Microbiol       Date:  2011-09-23       Impact factor: 4.792

6.  Identification and characterization of the Bacillus thuringiensis phaZ gene, encoding new intracellular poly-3-hydroxybutyrate depolymerase.

Authors:  Chi-Ling Tseng; Hui-Ju Chen; Gwo-Chyuan Shaw
Journal:  J Bacteriol       Date:  2006-08-25       Impact factor: 3.490

7.  To be or not to be a poly(3-hydroxybutyrate) (PHB) depolymerase: PhaZd1 (PhaZ6) and PhaZd2 (PhaZ7) of Ralstonia eutropha, highly active PHB depolymerases with no detectable role in mobilization of accumulated PHB.

Authors:  Anna Sznajder; Dieter Jendrossek
Journal:  Appl Environ Microbiol       Date:  2014-06-06       Impact factor: 4.792

8.  Microbial functional diversity plays an important role in the degradation of polyhydroxybutyrate (PHB) in soil.

Authors:  Samrat Dey; Prosun Tribedi
Journal:  3 Biotech       Date:  2018-03-09       Impact factor: 2.406

9.  PhaM is the physiological activator of poly(3-hydroxybutyrate) (PHB) synthase (PhaC1) in Ralstonia eutropha.

Authors:  Daniel Pfeiffer; Dieter Jendrossek
Journal:  Appl Environ Microbiol       Date:  2013-11-08       Impact factor: 4.792

10.  Isolated poly(3-hydroxybutyrate) (PHB) granules are complex bacterial organelles catalyzing formation of PHB from acetyl coenzyme A (CoA) and degradation of PHB to acetyl-CoA.

Authors:  Keiichi Uchino; Terumi Saito; Birgit Gebauer; Dieter Jendrossek
Journal:  J Bacteriol       Date:  2007-08-24       Impact factor: 3.490
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