Literature DB >> 15988060

Purification of PDE6 isozymes from mammalian retina.

Dana C Pentia1, Suzanne Hosier, Rachel A Collupy, Beverly A Valeriani, Rick H Cote.   

Abstract

The photoreceptor phosphodiesterase (PDE6) is the central effector of visual transduction in vertebrate retinal photoreceptors. Distinct isozymes of PDE6 exist in rods and cones. Mammalian retina serves as an abundant source of tissue for PDE6 purification. Methods are described for the isolation and purification of membrane-associated PDE6 from rod outer segment membranes. Purification of cone PDE6 from the soluble fraction of retinal extracts is also described. Several procedures that can purify the rod and cone isozymes to homogeneity, including anion exchange, hydrophobic interaction, gel filtration, hydroxyapatite, and immunoaffinity chromatography, are presented. A method to activate PDE6 by limited proteolysis of its inhibitory gamma-subunit is also provided.

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Year:  2005        PMID： 15988060     DOI： 10.1385/1-59259-839-0:125

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Characterization of conformational changes and protein-protein interactions of rod photoreceptor phosphodiesterase (PDE6).

Authors:  Suzanne L Matte; Thomas M Laue; Rick H Cote
Journal:  J Biol Chem       Date:  2012-04-18       Impact factor: 5.157

2.  Functional mapping of interacting regions of the photoreceptor phosphodiesterase (PDE6) γ-subunit with PDE6 catalytic dimer, transducin, and regulator of G-protein signaling9-1 (RGS9-1).

Authors:  Xiu-Jun Zhang; Xiong-Zhuo Gao; Wei Yao; Rick H Cote
Journal:  J Biol Chem       Date:  2012-06-04       Impact factor: 5.157

3.  Evaluation of the 17-kDa prenyl-binding protein as a regulatory protein for phototransduction in retinal photoreceptors.

Authors:  Angela W Norton; Suzanne Hosier; Jennifer M Terew; Ning Li; Anuradha Dhingra; Noga Vardi; Wolfgang Baehr; Rick H Cote
Journal:  J Biol Chem       Date:  2004-10-25       Impact factor: 5.157

4.  Direct allosteric regulation between the GAF domain and catalytic domain of photoreceptor phosphodiesterase PDE6.

Authors:  Xiu-Jun Zhang; Karyn B Cahill; Arye Elfenbein; Vadim Y Arshavsky; Rick H Cote
Journal:  J Biol Chem       Date:  2008-09-08       Impact factor: 5.157

5.  Probing the catalytic sites and activation mechanism of photoreceptor phosphodiesterase using radiolabeled phosphodiesterase inhibitors.

Authors:  Yu-Ting Liu; Suzanne L Matte; Jackie D Corbin; Sharron H Francis; Rick H Cote
Journal:  J Biol Chem       Date:  2009-09-16       Impact factor: 5.157

6.  Structural requirements of the photoreceptor phosphodiesterase gamma-subunit for inhibition of rod PDE6 holoenzyme and for its activation by transducin.

Authors:  Xiu-Jun Zhang; Nikolai P Skiba; Rick H Cote
Journal:  J Biol Chem       Date:  2009-11-30       Impact factor: 5.157

7.  The N termini of the inhibitory γ-subunits of phosphodiesterase-6 (PDE6) from rod and cone photoreceptors differentially regulate transducin-mediated PDE6 activation.

Authors:  Xin Wang; David C Plachetzki; Rick H Cote
Journal:  J Biol Chem       Date:  2019-04-08       Impact factor: 5.157

8.  Molecular architecture of photoreceptor phosphodiesterase elucidated by chemical cross-linking and integrative modeling.

Authors:  Xiaohui Zeng-Elmore; Xiong-Zhuo Gao; Riccardo Pellarin; Dina Schneidman-Duhovny; Xiu-Jun Zhang; Katie A Kozacka; Yang Tang; Andrej Sali; Robert J Chalkley; Rick H Cote; Feixia Chu
Journal:  J Mol Biol       Date:  2014-08-19       Impact factor: 5.469

9.  The molecular architecture of photoreceptor phosphodiesterase 6 (PDE6) with activated G protein elucidates the mechanism of visual excitation.

Authors:  Michael J Irwin; Richa Gupta; Xiong-Zhuo Gao; Karyn B Cahill; Feixia Chu; Rick H Cote
Journal:  J Biol Chem       Date:  2019-11-05       Impact factor: 5.157

10.  Allosteric Regulation of Rod Photoreceptor Phosphodiesterase 6 (PDE6) Elucidated by Chemical Cross-Linking and Quantitative Mass Spectrometry.

Authors:  Feixia Chu; Donna Hogan; Richa Gupta; Xiong-Zhuo Gao; Hieu T Nguyen; Rick H Cote
Journal:  J Mol Biol       Date:  2019-08-05       Impact factor: 5.469
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