| Literature DB >> 15950270 |
Takehito Kaneko1, Stefan Moisyadi, Ryota Suganuma, Barbara Hohn, Ryuzo Yanagimachi, Pawel Pelczar.
Abstract
The low efficiency of current microinjection-based animal transgenesis techniques is largely the result of poor embryo survival. We have developed a new, bacterial recombinase-based transgenesis method. Intracytoplasmic sperm injection (ICSI) of single stranded DNA (ssDNA) complexed with E. coli recombinase RecA into mouse metaphaseII (MII) arrested oocytes resulted in RecA-dependent transgenesis. This approach offers significant advantages over pronuclear microinjection and previous ICSI-based transgenesis approaches in terms of improved embryo survival, which translates into greater transgenesis efficiency. It also opens the possibility to attempt experiments, which may affect gene targeting by homologous recombination into DNA of mammalian single celled pre-implantation embryos.Entities:
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Year: 2005 PMID： 15950270 DOI： 10.1016/j.theriogenology.2005.04.003
Source DB: PubMed Journal: Theriogenology ISSN： 0093-691X Impact factor: 2.740