Literature DB >> 15935500

A giant step towards artificial life?

David Deamer1.   

Abstract

Step by step, the components of an artificial form of cellular life are being assembled by researchers. Lipid vesicles the size of small bacteria can be prepared and under certain conditions are able to grow and divide, then grow again. Polymerase enzymes encapsulated in the vesicles can synthesize RNA from externally added substrates. Most recently, the entire translation apparatus, including ribosomes, has been captured in vesicles. Substantial amounts of proteins were produced, including green fluorescent protein used as a marker for protein synthesis. Can we now assemble a living cell? Not quite yet because no one has produced a polymerase that can be reproduced along with growth of the other molecular components required by life. But we are closer than ever before.

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Year:  2005        PMID: 15935500     DOI: 10.1016/j.tibtech.2005.05.008

Source DB:  PubMed          Journal:  Trends Biotechnol        ISSN: 0167-7799            Impact factor:   19.536


  22 in total

Review 1.  Constructing partial models of cells.

Authors:  Norikazu Ichihashi; Tomoaki Matsuura; Hiroshi Kita; Takeshi Sunami; Hiroaki Suzuki; Tetsuya Yomo
Journal:  Cold Spring Harb Perspect Biol       Date:  2010-04-28       Impact factor: 10.005

Review 2.  Biology by design: reduction and synthesis of cellular components and behaviour.

Authors:  Philippe Marguet; Frederick Balagadde; Cheemeng Tan; Lingchong You
Journal:  J R Soc Interface       Date:  2007-08-22       Impact factor: 4.118

3.  Synthetic Turing protocells: vesicle self-reproduction through symmetry-breaking instabilities.

Authors:  Javier Macía; Ricard V Solé
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2007-10-29       Impact factor: 6.237

4.  Question 8: bridging the gap between in silico and in vitro approaches to minimal cells.

Authors:  Fabio Mavelli; Kepa Ruiz-Mirazo
Journal:  Orig Life Evol Biosph       Date:  2007-06-28       Impact factor: 1.950

Review 5.  Synthetic protocell biology: from reproduction to computation.

Authors:  Ricard V Solé; Andreea Munteanu; Carlos Rodriguez-Caso; Javier Macía
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2007-10-29       Impact factor: 6.237

6.  Piecing together a puzzle. An exposition of synthetic biology.

Authors:  Anna Deplazes
Journal:  EMBO Rep       Date:  2009-05       Impact factor: 8.807

7.  It's life, but just as we know it.

Authors:  Mark Greener
Journal:  EMBO Rep       Date:  2008-11       Impact factor: 8.807

8.  Sugar synthesis in a protocellular model leads to a cell signalling response in bacteria.

Authors:  Paul M Gardner; Klaus Winzer; Benjamin G Davis
Journal:  Nat Chem       Date:  2009-07-24       Impact factor: 24.427

9.  Chemical biology: How to sweet-talk bacteria.

Authors:  Geoffrey J T Cooper; Leroy Cronin
Journal:  Nat Chem       Date:  2009-08       Impact factor: 24.427

10.  Vesicles-on-a-chip: A universal microfluidic platform for the assembly of liposomes and polymersomes.

Authors:  Julien Petit; Ingmar Polenz; Jean-Christophe Baret; Stephan Herminghaus; Oliver Bäumchen
Journal:  Eur Phys J E Soft Matter       Date:  2016-06-14       Impact factor: 1.890

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