Using end-labelled RNA, significant changes in base specificity of three nucleases have been detected under defined conditions. Staphylococcus aureus nuclease at pH 3.5 without Ca++ cleaves all Pyr-N bonds more uniformly and efficiently than RNase A, without any preference for Pyr-A bonds. At pH 7.5 in 10 mM Ca++ this enzyme cleaves all N-C and N-G bonds slowly, whereas N-U and N-A bonds are hydrolyzed rapidly. Hence, the base at the 3'- or at the 5'-side of a phosphodiester bond can determine the base specificity of S. aureus nuclease. - In absence of urea, Neurospora crassa endonuclease cleaves all phosphodiester bonds, but leaves all C-N bonds intact in 7 M urea. - RNase U2 at pH 3.5 cleaves A-N bonds more efficiently than at pH 5.0.
Using end-labelled RNA, significant changes in base specificity of three nucleases have been detected under defined conditions. Staphylococcus aureus nuclease at pH 3.5 without Ca++ cleaves all n class="Chemical">Pyr-N bonds more uniformly and efficiently than RNase A, without any preference for Pyr-A bonds. At pH 7.5 in 10 mM Ca++ this enzyme cleaves all N-C and N-G bonds slowly, whereas N-U and N-A bonds are hydrolyzed rapidly. Hence, the base at the 3'- or at the 5'-side of a phosphodiester bond can determine the base specificity of S. aureus nuclease. - In absence of urea, Neurospora crassa endonuclease cleaves all phosphodiester bonds, but leaves all C-N bonds intact in 7 M urea. - RNase U2 at pH 3.5 cleaves A-N bonds more efficiently than at pH 5.0.
Authors: R E Lockard; B Alzner-Deweerd; J E Heckman; J MacGee; M W Tabor; U L RajBhandary Journal: Nucleic Acids Res Date: 1978-01 Impact factor: 16.971
Authors: E C Koper-Zwarthoff; R E Lockard; B Alzner-deWeerd; U L RajBhandary; J F Bol Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205
Authors: C Branlant; A Krol; J P Ebel; E Lazar; H Gallinaro; M Jacob; J Sri-Widada; P Jeanteur Journal: Nucleic Acids Res Date: 1980-09-25 Impact factor: 16.971