Literature DB >> 15659662

Identification and characterization of genes involved in the downstream degradation pathway of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis UT26.

Ryo Endo1, Mayuko Kamakura, Keisuke Miyauchi, Masao Fukuda, Yoshiyuki Ohtsubo, Masataka Tsuda, Yuji Nagata.   

Abstract

Sphingomonas paucimobilis UT26 utilizes gamma-hexachlorocyclohexane (gamma-HCH) as a sole source of carbon and energy. In our previous study, we cloned and characterized genes that are involved in the conversion of gamma-HCH to maleylacetate (MA) via chlorohydroquinone (CHQ) in UT26. In this study, we identified and characterized an MA reductase gene, designated linF, that is essential for the utilization of gamma-HCH in UT26. A gene named linEb, whose deduced product showed significant identity to LinE (53%), was located close to linF. LinE is a novel type of ring cleavage dioxygenase that catalyzes the conversion of CHQ to MA. LinEb expressed in Escherichia coli transformed CHQ and 2,6-dichlorohydroquinone to MA and 2-chloromaleylacetate, respectively. Our previous and present results indicate that UT26 (i) has two gene clusters for degradation of chlorinated aromatic compounds via hydroquinone-type intermediates and (ii) uses at least parts of both clusters for gamma-HCH utilization.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 15659662      PMCID： PMC545726          DOI： 10.1128/JB.187.3.847-853.2005

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  36 in total

1.  Different types of dienelactone hydrolase in 4-fluorobenzoate-utilizing bacteria.

Authors:  M Schlömann; E Schmidt; H J Knackmuss
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

2.  Evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway.

Authors:  T Kasberg; D L Daubaras; A M Chakrabarty; D Kinzelt; W Reineke
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490

3.  Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis.

Authors:  H D Schweizer
Journal:  Biotechniques       Date:  1993-11       Impact factor: 1.993

4.  PcpA, which is involved in the degradation of pentachlorophenol in Sphingomonas chlorophenolica ATCC39723, is a novel type of ring-cleavage dioxygenase.

Authors:  Y Ohtsubo; K Miyauchi; K Kanda; T Hatta; H Kiyohara; T Senda; Y Nagata; Y Mitsui; M Takagi
Journal:  FEBS Lett       Date:  1999-10-15       Impact factor: 4.124

5.  Cloning and sequencing of two tandem genes involved in degradation of 2,3-dihydroxybiphenyl to benzoic acid in the polychlorinated biphenyl-degrading soil bacterium Pseudomonas sp. strain KKS102.

Authors:  K Kimbara; T Hashimoto; M Fukuda; T Koana; M Takagi; M Oishi; K Yano
Journal:  J Bacteriol       Date:  1989-05       Impact factor: 3.490

6.  The tfdR gene product can successfully take over the role of the insertion element-inactivated TfdT protein as a transcriptional activator of the tfdCDEF gene cluster, which encodes chlorocatechol degradation in Ralstonia eutropha JMP134(pJP4)

Authors:  J H Leveau; J R van der Meer
Journal:  J Bacteriol       Date:  1996-12       Impact factor: 3.490

7.  Organization and regulation of pentachlorophenol-degrading genes in Sphingobium chlorophenolicum ATCC 39723.

Authors:  Mian Cai; Luying Xun
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

8.  Distribution and organization of auxotrophic genes on the multichromosomal genome of Burkholderia multivorans ATCC 17616.

Authors:  Harunobu Komatsu; Yoshiyuki Imura; Akira Ohori; Yuji Nagata; Masataka Tsuda
Journal:  J Bacteriol       Date:  2003-06       Impact factor: 3.490

9.  A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants.

Authors:  T T Hoang; R R Karkhoff-Schweizer; A J Kutchma; H P Schweizer
Journal:  Gene       Date:  1998-05-28       Impact factor: 3.688

10.  Bacterial metabolism of 4-chlorophenoxyacetate.

Authors:  W C Evans; B S Smith; P Moss; H N Fernley
Journal:  Biochem J       Date:  1971-05       Impact factor: 3.857
View more
  21 in total

1.  Determination of the active site of Sphingobium chlorophenolicum 2,6-dichlorohydroquinone dioxygenase (PcpA).

Authors:  Timothy E Machonkin; Patrick L Holland; Kristine N Smith; Justin S Liberman; Adriana Dinescu; Thomas R Cundari; Sara S Rocks
Journal:  J Biol Inorg Chem       Date:  2010-03       Impact factor: 3.358

Review 2.  How enzyme promiscuity and horizontal gene transfer contribute to metabolic innovation.

Authors:  Margaret E Glasner; Dat P Truong; Benjamin C Morse
Journal:  FEBS J       Date:  2020-01-10       Impact factor: 5.542

3.  Two rhizobial strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, encode haloalkane dehalogenases with novel structures and substrate specificities.

Authors:  Yukari Sato; Marta Monincová; Radka Chaloupková; Zbynek Prokop; Yoshiyuki Ohtsubo; Kiwamu Minamisawa; Masataka Tsuda; Jirí Damborsky; Yuji Nagata
Journal:  Appl Environ Microbiol       Date:  2005-08       Impact factor: 4.792

4.  Complete genome sequence of the representative γ-hexachlorocyclohexane-degrading bacterium Sphingobium japonicum UT26.

Authors:  Yuji Nagata; Yoshiyuki Ohtsubo; Ryo Endo; Natsuko Ichikawa; Akiho Ankai; Akio Oguchi; Shigehiro Fukui; Nobuyuki Fujita; Masataka Tsuda
Journal:  J Bacteriol       Date:  2010-09-03       Impact factor: 3.490

5.  Complete nucleotide sequence of an exogenously isolated plasmid, pLB1, involved in gamma-hexachlorocyclohexane degradation.

Authors:  Ryo Miyazaki; Yukari Sato; Michihiro Ito; Yoshiyuki Ohtsubo; Yuji Nagata; Masataka Tsuda
Journal:  Appl Environ Microbiol       Date:  2006-09-08       Impact factor: 4.792

6.  2-haloacrylate hydratase, a new class of flavoenzyme that catalyzes the addition of water to the substrate for dehalogenation.

Authors:  Amr M Mowafy; Tatsuo Kurihara; Atsushi Kurata; Tadashi Uemura; Nobuyoshi Esaki
Journal:  Appl Environ Microbiol       Date:  2010-07-23       Impact factor: 4.792

7.  Metabolic pathway involved in 2-methyl-6-ethylaniline degradation by Sphingobium sp. strain MEA3-1 and cloning of the novel flavin-dependent monooxygenase system meaBA.

Authors:  Weiliang Dong; Qiongzhen Chen; Ying Hou; Shuhuan Li; Kai Zhuang; Fei Huang; Jie Zhou; Zhoukun Li; Jue Wang; Lei Fu; Zhengguang Zhang; Yan Huang; Fei Wang; Zhongli Cui
Journal:  Appl Environ Microbiol       Date:  2015-09-18       Impact factor: 4.792

8.  Crystallization and preliminary X-ray analysis of gamma-hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26.

Authors:  Masahiko Okai; Keiko Kubota; Masao Fukuda; Yuji Nagata; Koji Nagata; Masaru Tanokura
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-07-30

9.  Identification and characterization of genes encoding a putative ABC-type transporter essential for utilization of gamma-hexachlorocyclohexane in Sphingobium japonicum UT26.

Authors:  Ryo Endo; Yoshiyuki Ohtsubo; Masataka Tsuda; Yuji Nagata
Journal:  J Bacteriol       Date:  2007-03-16       Impact factor: 3.490

10.  Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA.

Authors:  Yoshihiko Nanasato; Sayuri Namiki; Masao Oshima; Ryota Moriuchi; Ken-Ichi Konagaya; Nobuyasu Seike; Takashi Otani; Yuji Nagata; Masataka Tsuda; Yutaka Tabei
Journal:  Plant Cell Rep       Date:  2016-06-13       Impact factor: 4.570
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.