Literature DB >> 19652349

Crystallization and preliminary X-ray analysis of gamma-hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26.

Masahiko Okai1, Keiko Kubota, Masao Fukuda, Yuji Nagata, Koji Nagata, Masaru Tanokura.   

Abstract

LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from gamma-hexachlorocyclohexane (gamma-HCH) to 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN) via gamma-pentachlorocyclohexene (gamma-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P4(1) or P4(3), with unit-cell parameters a = b = 68.9, c = 101.9 A, and diffracted X-rays to 2.25 A resolution. The crystal contained three molecules in the asymmetric unit.

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Year:  2009        PMID： 19652349      PMCID： PMC2720343          DOI： 10.1107/S1744309109026645

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  17 in total

1.  Reaction mechanism and stereochemistry of gamma-hexachlorocyclohexane dehydrochlorinase LinA.

Authors:  L Trantírek; K Hynková; Y Nagata; A Murzin; A Ansorgová; V Sklenár; J Damborský
Journal:  J Biol Chem       Date:  2000-11-30       Impact factor: 5.157

2.  [Isolation and characterization of a HCH degradation Sphingomanas sp. stain BHC-A].

Authors:  Ai-zhi Ma; Jun Wu; Guo-shun Zhang; Ting Wang; Shun-peng Li
Journal:  Wei Sheng Wu Xue Bao       Date:  2005-10

3.  Distribution and phylogeny of hexachlorocyclohexane-degrading bacteria in soils from Spain.

Authors:  William W Mohn; Birgit Mertens; Josh D Neufeld; Willy Verstraete; Victor de Lorenzo
Journal:  Environ Microbiol       Date:  2006-01       Impact factor: 5.491

4.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

5.  Complete analysis of genes and enzymes for gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26.

Authors:  Y Nagata; K Miyauchi; M Takagi
Journal:  J Ind Microbiol Biotechnol       Date:  1999-10       Impact factor: 3.346

6.  Cloning and characterization of lin genes responsible for the degradation of Hexachlorocyclohexane isomers by Sphingomonas paucimobilis strain B90.

Authors:  Rekha Kumari; Sanjukta Subudhi; Mrutyunjay Suar; Gauri Dhingra; Vishakha Raina; Charu Dogra; Sukanya Lal; Jan Roelof van der Meer; Christof Holliger; Rup Lal
Journal:  Appl Environ Microbiol       Date:  2002-12       Impact factor: 4.792

7.  Organization of lin genes and IS6100 among different strains of hexachlorocyclohexane-degrading Sphingomonas paucimobilis: evidence for horizontal gene transfer.

Authors:  Charu Dogra; Vishakha Raina; Rinku Pal; Mrutyunjay Suar; Sukanya Lal; Karl-Heinz Gartemann; Christof Holliger; Jan Roelof van der Meer; Rup Lal
Journal:  J Bacteriol       Date:  2004-04       Impact factor: 3.490

8.  Plasmid-encoded gamma-hexachlorocyclohexane degradation genes and insertion sequences in Sphingobium francense (ex-Sphingomonas paucimobilis Sp+).

Authors:  Hélène Cérémonie; Hasna Boubakri; Patrick Mavingui; Pascal Simonet; Timothy M Vogel
Journal:  FEMS Microbiol Lett       Date:  2006-04       Impact factor: 2.742

9.  Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase.

Authors:  B Kauppi; K Lee; E Carredano; R E Parales; D T Gibson; H Eklund; S Ramaswamy
Journal:  Structure       Date:  1998-05-15       Impact factor: 5.006

Review 10.  Aerobic degradation of lindane (gamma-hexachlorocyclohexane) in bacteria and its biochemical and molecular basis.

Authors:  Yuji Nagata; Ryo Endo; Michihiro Ito; Yoshiyuki Ohtsubo; Masataka Tsuda
Journal:  Appl Microbiol Biotechnol       Date:  2007-07-19       Impact factor: 4.813
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  3 in total

Review 1.  Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation.

Authors:  Rup Lal; Gunjan Pandey; Pooja Sharma; Kirti Kumari; Shweta Malhotra; Rinku Pandey; Vishakha Raina; Hans-Peter E Kohler; Christof Holliger; Colin Jackson; John G Oakeshott
Journal:  Microbiol Mol Biol Rev       Date:  2010-03       Impact factor: 11.056

2.  FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants.

Authors:  David Bednar; Koen Beerens; Eva Sebestova; Jaroslav Bendl; Sagar Khare; Radka Chaloupkova; Zbynek Prokop; Jan Brezovsky; David Baker; Jiri Damborsky
Journal:  PLoS Comput Biol       Date:  2015-11-03       Impact factor: 4.475

3.  Genomic Analysis of γ-Hexachlorocyclohexane-Degrading Sphingopyxis lindanitolerans WS5A3p Strain in the Context of the Pangenome of Sphingopyxis.

Authors:  Michal A Kaminski; Adam Sobczak; Andrzej Dziembowski; Leszek Lipinski
Journal:  Genes (Basel)       Date:  2019-09-06       Impact factor: 4.096
  3 in total

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