Literature DB >> 15649759

A selective experiment for the sequential protein backbone assignment from 3D heteronuclear spectra.

Wolfgang Bermel1, Ivano Bertini, Isabella C Felli, Roberta Pierattelli, Paul R Vasos.   

Abstract

Two modifications of the triple-resonance CANCO sequence, designed for backbone assignment in proteins [Angew. Chem. Int. Ed. 43 (2004) 2257], are presented here. These two new sequences display the intra-residue Ca-CO correlation selectively, while in the original sequence both the inter- and the intra-residue correlations were present. In addition, one of the two variants benefits from an improved sensitivity. Both sequences are a useful complement to the CANCO sequence for facile sequence-specific protein assignment by protonless NMR.

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Year:  2005        PMID: 15649759     DOI: 10.1016/j.jmr.2004.11.005

Source DB:  PubMed          Journal:  J Magn Reson        ISSN: 1090-7807            Impact factor:   2.229


  9 in total

1.  Detection of disordered regions in globular proteins using ¹³C-detected NMR.

Authors:  Felicia L V Gray; Marcelo J Murai; Jolanta Grembecka; Tomasz Cierpicki
Journal:  Protein Sci       Date:  2012-12       Impact factor: 6.725

2.  Improving the chemical shift dispersion of multidimensional NMR spectra of intrinsically disordered proteins.

Authors:  Wolfgang Bermel; Marta Bruix; Isabella C Felli; Vasantha Kumar M V; Roberta Pierattelli; Soraya Serrano
Journal:  J Biomol NMR       Date:  2013-01-12       Impact factor: 2.835

3.  Relaxation-optimised Hartmann-Hahn transfer using a specifically Tailored MOCCA-XY16 mixing sequence for carbonyl-carbonyl correlation spectroscopy in 13C direct detection NMR experiments.

Authors:  Isabella C Felli; Roberta Pierattelli; Steffen J Glaser; Burkhard Luy
Journal:  J Biomol NMR       Date:  2009-02-18       Impact factor: 2.835

4.  Assignment strategy for fast relaxing signals: complete aminoacid identification in thulium substituted calbindin D 9K.

Authors:  Stéphane Balayssac; Beatriz Jiménez; Mario Piccioli
Journal:  J Biomol NMR       Date:  2006-02       Impact factor: 2.835

5.  Completing the circuit: direct-observe 13C,15N double-quantum spectroscopy permits sequential resonance assignments near a paramagnetic center in acireductone dioxygenase.

Authors:  Susan Sondej Pochapsky; Joel C Sunshine; Thomas C Pochapsky
Journal:  J Am Chem Soc       Date:  2008-01-30       Impact factor: 15.419

6.  Selective backbone labeling of proteins using 1,2-13C2-pyruvate as carbon source.

Authors:  Chenyun Guo; Chun Geng; Vitali Tugarinov
Journal:  J Biomol NMR       Date:  2009-05-26       Impact factor: 2.835

7.  13C-detected NMR experiments for measuring chemical shifts and coupling constants in nucleic acid bases.

Authors:  Radovan Fiala; Vladimír Sklenár
Journal:  J Biomol NMR       Date:  2007-08-14       Impact factor: 2.582

Review 8.  13C Direct Detected NMR for Challenging Systems.

Authors:  Isabella C Felli; Roberta Pierattelli
Journal:  Chem Rev       Date:  2022-01-13       Impact factor: 72.087

9.  1H, 13C and 15N resonance assignment of the SARS-CoV-2 full-length nsp1 protein and its mutants reveals its unique secondary structure features in solution.

Authors:  Tatiana Agback; Francisco Dominguez; Ilya Frolov; Elena I Frolova; Peter Agback
Journal:  PLoS One       Date:  2021-12-07       Impact factor: 3.752

  9 in total

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