Literature DB >> 15640243

High-level overproduction of His-tagged Tth DNA polymerase in Thermus thermophilus.

Renata Moreno1, Amparo Haro, Angel Castellanos, José Berenguer.   

Abstract

A new plasmid for the overexpression of His-tagged thermozymes in Thermus thermophilus was developed. With this plasmid, soluble and active histidine-tagged DNA polymerase from T. thermophilus was overproduced in larger amounts in the thermophile than in Escherichia coli. The protein purified from the thermophile was active in PCR.

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Year:  2005        PMID： 15640243      PMCID： PMC544263          DOI： 10.1128/AEM.71.1.591-593.2005

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

Review 1.  Biotechnologically relevant enzymes from Thermus thermophilus.

Authors:  A A Pantazaki; A A Pritsa; D A Kyriakidis
Journal:  Appl Microbiol Biotechnol       Date:  2002-01       Impact factor: 4.813

Review 2.  The enzymes from extreme thermophiles: bacterial sources, thermostabilities and industrial relevance.

Authors:  T Coolbear; R M Daniel; H W Morgan
Journal:  Adv Biochem Eng Biotechnol       Date:  1992       Impact factor: 2.635

Review 3.  Finding and using hyperthermophilic enzymes.

Authors:  M W Adams; R M Kelly
Journal:  Trends Biotechnol       Date:  1998-08       Impact factor: 19.536

4.  Vectors for selective expression of cloned DNAs by T7 RNA polymerase.

Authors:  A H Rosenberg; B N Lade; D S Chui; S W Lin; J J Dunn; F W Studier
Journal:  Gene       Date:  1987       Impact factor: 3.688

5.  Thermus thermophilus as a cell factory for the production of a thermophilic Mn-dependent catalase which fails to be synthesized in an active form in Escherichia coli.

Authors:  Aurelio Hidalgo; Lorena Betancor; Renata Moreno; Olga Zafra; Felipe Cava; Roberto Fernández-Lafuente; José M Guisán; José Berenguer
Journal:  Appl Environ Microbiol       Date:  2004-07       Impact factor: 4.792

6.  Heterologous gene expression in Thermus thermophilus: beta-galactosidase, dibenzothiophene monooxygenase, PNB carboxy esterase, 2-aminobiphenyl-2,3-diol dioxygenase, and chloramphenicol acetyl transferase.

Authors:  Ho-Shin Park; Kevin J Kayser; Jung-Ho Kwak; John J Kilbane
Journal:  J Ind Microbiol Biotechnol       Date:  2004-05-12       Impact factor: 3.346

7.  Development of Thermus-Escherichia shuttle vectors and their use for expression of the Clostridium thermocellum celA gene in Thermus thermophilus.

Authors:  I Lasa; M de Grado; M A de Pedro; J Berenguer
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

8.  A plasmid vector for an extreme thermophile, Thermus thermophilus.

Authors:  Y Koyama; Y Arikawa; K Furukawa
Journal:  FEMS Microbiol Lett       Date:  1990-10       Impact factor: 2.742

9.  The genome sequence of the extreme thermophile Thermus thermophilus.

Authors:  Anke Henne; Holger Brüggemann; Carsten Raasch; Arnim Wiezer; Thomas Hartsch; Heiko Liesegang; Andre Johann; Tanja Lienard; Olivia Gohl; Rosa Martinez-Arias; Carsten Jacobi; Vytaute Starkuviene; Silke Schlenczeck; Silke Dencker; Robert Huber; Hans-Peter Klenk; Wilfried Kramer; Rainer Merkl; Gerhard Gottschalk; Hans-Joachim Fritz
Journal:  Nat Biotechnol       Date:  2004-04-04       Impact factor: 54.908

10.  Development of plasmid cloning vectors for Thermus thermophilus HB8: expression of a heterologous, plasmid-borne kanamycin nucleotidyltransferase gene.

Authors:  M W Mather; J A Fee
Journal:  Appl Environ Microbiol       Date:  1992-01       Impact factor: 4.792
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  13 in total

1.  Identification of a replication initiation protein of the pVV8 plasmid from Thermus thermophilus HB8.

Authors:  Naoto Ohtani; Masaru Tomita; Mitsuhiro Itaya
Journal:  Extremophiles       Date:  2012-11-01       Impact factor: 2.395

2.  Polysaccharide-degrading thermophiles generated by heterologous gene expression in Geobacillus kaustophilus HTA426.

Authors:  Hirokazu Suzuki; Ken-ichi Yoshida; Toshihisa Ohshima
Journal:  Appl Environ Microbiol       Date:  2013-06-21       Impact factor: 4.792

3.  Phosphoproteomic analysis reveals the effects of PilF phosphorylation on type IV pilus and biofilm formation in Thermus thermophilus HB27.

Authors:  Wan-Ling Wu; Jiahn-Haur Liao; Guang-Huey Lin; Miao-Hsia Lin; Ying-Che Chang; Suh-Yuen Liang; Feng-Ling Yang; Kay-Hooi Khoo; Shih-Hsiung Wu
Journal:  Mol Cell Proteomics       Date:  2013-07-04       Impact factor: 5.911

4.  Characterization of the PIB-Type ATPases present in Thermus thermophilus.

Authors:  Lici A Schurig-Briccio; Robert B Gennis
Journal:  J Bacteriol       Date:  2012-05-25       Impact factor: 3.490

Review 5.  Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms.

Authors:  James A Counts; Benjamin M Zeldes; Laura L Lee; Christopher T Straub; Michael W W Adams; Robert M Kelly
Journal:  Wiley Interdiscip Rev Syst Biol Med       Date:  2017-02-16

6.  Omp85(Tt) from Thermus thermophilus HB27: an ancestral type of the Omp85 protein family.

Authors:  Jutta Nesper; Alexander Brosig; Philippe Ringler; Geetika J Patel; Shirley A Müller; Jörg H Kleinschmidt; Winfried Boos; Kay Diederichs; Wolfram Welte
Journal:  J Bacteriol       Date:  2008-05-02       Impact factor: 3.490

Review 7.  Galactomannan degradation by thermophilic enzymes: a hot topic for biotechnological applications.

Authors:  Martina Aulitto; Salvatore Fusco; Danila Limauro; Gabriella Fiorentino; Simonetta Bartolucci; Patrizia Contursi
Journal:  World J Microbiol Biotechnol       Date:  2019-01-30       Impact factor: 3.312

8.  Characterization of three putative Lon proteases of Thermus thermophilus HB27 and use of their defective mutants as hosts for production of heterologous proteins.

Authors:  Tomoko Maehara; Takayuki Hoshino; Akira Nakamura
Journal:  Extremophiles       Date:  2007-12-22       Impact factor: 2.395

Review 9.  Thermus thermophilus as biological model.

Authors:  Felipe Cava; Aurelio Hidalgo; José Berenguer
Journal:  Extremophiles       Date:  2009-01-21       Impact factor: 2.395

10.  Importance of the latex-clearing protein (Lcp) for poly(cis-1,4-isoprene) rubber cleavage in Streptomyces sp. K30.

Authors:  Meral Yikmis; Alexander Steinbüchel
Journal:  Microbiologyopen       Date:  2012-03       Impact factor: 3.139
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