BACKGROUND: The aim of this study was to evaluate a new approach for killing periodontopathogenic bacteria using photodynamic therapy (PDT). METHODS: In this study, we investigated the photosensitizers chlorin e6, BLC 1010, and BLC 1014 by three different methods for their effect in PDT on the viability of periodontopathogenic bacterial species. The methods included examination of inhibition zones on agar plates, determination of colony-forming units (CFU), and the use of a bacterial viability kit. RESULTS: Using the CFU method, we were able to demonstrate that the anaerobic bacteria Porphyromonas gingivalis, Fusobacterium nucleatum, and Capnocytophaga gingivalis can be photoinactivated completely by illumination with an intensity of 5.3 J/cm2 in the presence of 10 microM chlorin e6 and 10 microM BLC 1010. With the photosensitizers chlorin e6 and BLC 1010, we were able to induce zones of inhibition on agar plates. BLC 1014 failed to produce a zone of inhibition. The results of the bacterial viability test also showed that the photosensitizer BLC 1014 provides the lowest photodynamic effect in comparison to the others. CONCLUSION: The data collected to date suggest that photodynamic therapy with chlorin e6 and BLC 1010 is advantageous for suppressing periodontopathogenic bacteria.
BACKGROUND: The aim of this study was to evaluate a new approach for killing periodontopathogenic bacteria using photodynamic therapy (PDT). METHODS: In this study, we investigated the photosensitizers chlorin e6, BLC 1010, and BLC 1014 by three different methods for their effect in PDT on the viability of periodontopathogenic bacterial species. The methods included examination of inhibition zones on agar plates, determination of colony-forming units (CFU), and the use of a bacterial viability kit. RESULTS: Using the CFU method, we were able to demonstrate that the anaerobic bacteria Porphyromonas gingivalis, Fusobacterium nucleatum, and Capnocytophaga gingivalis can be photoinactivated completely by illumination with an intensity of 5.3 J/cm2 in the presence of 10 microM chlorin e6 and 10 microM BLC 1010. With the photosensitizers chlorin e6 and BLC 1010, we were able to induce zones of inhibition on agar plates. BLC 1014 failed to produce a zone of inhibition. The results of the bacterial viability test also showed that the photosensitizer BLC 1014 provides the lowest photodynamic effect in comparison to the others. CONCLUSION: The data collected to date suggest that photodynamic therapy with chlorin e6 and BLC 1010 is advantageous for suppressing periodontopathogenic bacteria.
Authors: Carlos de Paula Eduardo; Patricia Moreira de Freitas; Marcella Esteves-Oliveira; Ana Cecília Corrêa Aranha; Karen Müller Ramalho; Alyne Simões; Marina Stella Bello-Silva; Jan Tunér Journal: Lasers Med Sci Date: 2010-07-17 Impact factor: 3.161
Authors: Andreas Rühling; Jutta Fanghänel; Mohammad Houshmand; Alexander Kuhr; Peter Meisel; Christian Schwahn; Thomas Kocher Journal: Clin Oral Investig Date: 2009-10-13 Impact factor: 3.573
Authors: Anna Ossmann; Stefan Kranz; Guellmar Andre; Andrea Völpel; Volker Albrecht; Alfred Fahr; Bernd W Sigusch Journal: Clin Oral Investig Date: 2014-06-21 Impact factor: 3.573
Authors: Guilherme de Oliveira Macedo; Arthur B Novaes; Sérgio L S Souza; Mário Taba; Daniela B Palioto; Márcio F M Grisi Journal: Lasers Med Sci Date: 2013-03-10 Impact factor: 3.161