Literature DB >> 15528734

Molecular detection of linezolid resistance in Enterococcus faecium and Enterococcus faecalis by use of 5' nuclease real-time PCR compared to a modified classical approach.

Guido Werner1, Birgit Strommenger, Ingo Klare, Wolfgang Witte.   

Abstract

A nucleotide transversion from guanine to uracil in the 23S rRNA confers linezolid resistance. We describe a real-time PCR using two Taqman probes that detects a single mutated allele among the genomes of Enterococcus faecium and Enterococcus faecalis. Results were confirmed by a classical approach involving LabChip technology assayed with an Agilent Bioanalyzer 2100.

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Year:  2004        PMID： 15528734      PMCID： PMC525171          DOI： 10.1128/JCM.42.11.5327-5331.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  32 in total

1.  Linezolid breakpoints.

Authors: 
Journal:  Clin Microbiol Infect       Date:  2001-05       Impact factor: 8.067

Review 2.  Linezolid: a review of its use in the management of serious gram-positive infections.

Authors:  C M Perry; B Jarvis
Journal:  Drugs       Date:  2001       Impact factor: 9.546

3.  Infections due to vancomycin-resistant Enterococcus faecium resistant to linezolid.

Authors:  R D Gonzales; P C Schreckenberger; M B Graham; S Kelkar; K DenBesten; J P Quinn
Journal:  Lancet       Date:  2001-04-14       Impact factor: 79.321

4.  Linezolid resistance in a clinical isolate of Staphylococcus aureus.

Authors:  S Tsiodras; H S Gold; G Sakoulas; G M Eliopoulos; C Wennersten; L Venkataraman; R C Moellering; M J Ferraro
Journal:  Lancet       Date:  2001-07-21       Impact factor: 79.321

5.  Resistance to linezolid: characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci.

Authors:  J Prystowsky; F Siddiqui; J Chosay; D L Shinabarger; J Millichap; L R Peterson; G A Noskin
Journal:  Antimicrob Agents Chemother       Date:  2001-07       Impact factor: 5.191

6.  In vitro activities of linezolid against important gram-positive bacterial pathogens including vancomycin-resistant enterococci.

Authors:  G A Noskin; F Siddiqui; V Stosor; D Hacek; L R Peterson
Journal:  Antimicrob Agents Chemother       Date:  1999-08       Impact factor: 5.191

7.  Resistance mutations in 23 S rRNA identify the site of action of the protein synthesis inhibitor linezolid in the ribosomal peptidyl transferase center.

Authors:  P Kloss; L Xiong; D L Shinabarger; A S Mankin
Journal:  J Mol Biol       Date:  1999-11-19       Impact factor: 5.469

8.  Linezolid-resistant Enterococcus faecium isolated from a patient without prior exposure to an oxazolidinone: report from the SENTRY Antimicrobial Surveillance Program.

Authors:  Ronald N Jones; Phyllis Della-Latta; Lillian V Lee; Douglas J Biedenbach
Journal:  Diagn Microbiol Infect Dis       Date:  2002-02       Impact factor: 2.803

9.  Linezolid (PNU-100766) versus vancomycin in the treatment of hospitalized patients with nosocomial pneumonia: a randomized, double-blind, multicenter study.

Authors:  E Rubinstein; S Cammarata; T Oliphant; R Wunderink
Journal:  Clin Infect Dis       Date:  2001-01-26       Impact factor: 9.079

10.  Use of a LightCycler gyrA mutation assay for rapid identification of mutations conferring decreased susceptibility to ciprofloxacin in multiresistant Salmonella enterica serotype Typhimurium DT104 isolates.

Authors:  R A Walker; N Saunders; A J Lawson; E A Lindsay; M Dassama; L R Ward; M J Woodward; R H Davies; E Liebana; E J Threlfall
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948
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  9 in total

1.  Rapid emergence of resistance to linezolid during linezolid therapy of an Enterococcus faecium infection.

Authors:  Jamela Seedat; Günther Zick; Ingo Klare; Carola Konstabel; Norbert Weiler; Hany Sahly
Journal:  Antimicrob Agents Chemother       Date:  2006-09-18       Impact factor: 5.191

Review 2.  Spread of ampicillin/vancomycin-resistant Enterococcus faecium of the epidemic-virulent clonal complex-17 carrying the genes esp and hyl in German hospitals.

Authors:  I Klare; C Konstabel; S Mueller-Bertling; G Werner; B Strommenger; C Kettlitz; S Borgmann; B Schulte; D Jonas; A Serr; A M Fahr; U Eigner; W Witte
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2005-12       Impact factor: 3.267

3.  Detection of mutations conferring resistance to linezolid in Enterococcus spp. by fluorescence in situ hybridization.

Authors:  Guido Werner; Melanie Bartel; Nele Wellinghausen; Andreas Essig; Ingo Klare; Wolfgang Witte; Sven Poppert
Journal:  J Clin Microbiol       Date:  2007-05-02       Impact factor: 5.948

4.  Detection of new mutations conferring resistance to linezolid in glycopeptide-intermediate susceptibility Staphylococcus hominis subspecies hominis circulating in an intensive care unit.

Authors:  A Sorlozano; J Gutierrez; T Martinez; M E Yuste; J A Perez-Lopez; A Vindel; J Guillen; T Boquete
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2009-10-30       Impact factor: 3.267

5.  Use of pyrosequencing to identify point mutations in domain V of 23S rRNA genes of linezolid-resistant Staphylococcus aureus and Staphylococcus epidermidis.

Authors:  W Zhu; F C Tenover; J Limor; D Lonsway; D Prince; W M Dunne; J B Patel
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-03       Impact factor: 3.267

6.  Detection of a cfr(B) Variant in German Enterococcus faecium Clinical Isolates and the Impact on Linezolid Resistance in Enterococcus spp.

Authors:  Jennifer K Bender; Carola Fleige; Ingo Klare; Stefan Fiedler; Alexander Mischnik; Nico T Mutters; Kate E Dingle; Guido Werner
Journal:  PLoS One       Date:  2016-11-28       Impact factor: 3.240

7.  Validating a screening agar for linezolid-resistant enterococci.

Authors:  Guido Werner; Carola Fleige; Ingo Klare; Robert E Weber; Jennifer K Bender
Journal:  BMC Infect Dis       Date:  2019-12-23       Impact factor: 3.090

8.  Presence of optrA-mediated linezolid resistance in multiple lineages and plasmids of Enterococcus faecalis revealed by long read sequencing.

Authors:  Martin P McHugh; Benjamin J Parcell; Kerry A Pettigrew; Geoff Toner; Elham Khatamzas; Noha El Sakka; Anne Marie Karcher; Joanna Walker; Robert Weir; Danièle Meunier; Katie L Hopkins; Neil Woodford; Kate E Templeton; Stephen H Gillespie; Matthew T G Holden
Journal:  Microbiology (Reading)       Date:  2022-02       Impact factor: 2.777

9.  Prevalence of linezolid-resistant organisms among patients admitted to a tertiary hospital for critical care or dialysis.

Authors:  Kornelia Maria Dembicka; James Powell; Nuala H O'Connell; Noreen Hennessy; Grainne Brennan; Colum P Dunne
Journal:  Ir J Med Sci       Date:  2021-09-10       Impact factor: 2.089
  9 in total

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