Literature DB >> 15502236

Measuring recombination proficiency in mouse embryonic stem cells.

Andrew J Pierce1, Maria Jasin.   

Abstract

A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break. A fluorescence-based reporter is first gene targeted to the Hprt locus in a quantifiable way. A homing endonuclease expression vector is then introduced to generate a double-strand break, the repair of which is also quantifiable.

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Year:  2005        PMID： 15502236     DOI： 10.1385/1-59259-840-4:373

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  35 in total

1.  Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases.

Authors:  Lianne E M Vriend; Maria Jasin; Przemek M Krawczyk
Journal:  Methods Enzymol       Date:  2014       Impact factor: 1.600

2.  Homologous recombination conserves DNA sequence integrity throughout the cell cycle in embryonic stem cells.

Authors:  Lourdes Serrano; Li Liang; Yiming Chang; Li Deng; Christopher Maulion; Son Nguyen; Jay A Tischfield
Journal:  Stem Cells Dev       Date:  2010-10-29       Impact factor: 3.272

3.  Alternative pathways for the repair of RAG-induced DNA breaks.

Authors:  David M Weinstock; Maria Jasin
Journal:  Mol Cell Biol       Date:  2006-01       Impact factor: 4.272

4.  Assaying double-strand break repair pathway choice in mammalian cells using a targeted endonuclease or the RAG recombinase.

Authors:  David M Weinstock; Koji Nakanishi; Hildur R Helgadottir; Maria Jasin
Journal:  Methods Enzymol       Date:  2006       Impact factor: 1.600

5.  ING3 is required for ATM signaling and DNA repair in response to DNA double strand breaks.

Authors:  Audrey Mouche; Jérôme Archambeau; Charles Ricordel; Laura Chaillot; Nicolas Bigot; Thierry Guillaudeux; Muriel Grenon; Rémy Pedeux
Journal:  Cell Death Differ       Date:  2019-02-25       Impact factor: 15.828

6.  HDAC inhibitor PCI-24781 decreases RAD51 expression and inhibits homologous recombination.

Authors:  Shanthi Adimoolam; Mint Sirisawad; Jun Chen; Patti Thiemann; James M Ford; Joseph J Buggy
Journal:  Proc Natl Acad Sci U S A       Date:  2007-11-27       Impact factor: 11.205

7.  FBH1 helicase disrupts RAD51 filaments in vitro and modulates homologous recombination in mammalian cells.

Authors:  Jitka Simandlova; Jennifer Zagelbaum; Miranda J Payne; Wai Kit Chu; Igor Shevelev; Katsuhiro Hanada; Sujoy Chatterjee; Dylan A Reid; Ying Liu; Pavel Janscak; Eli Rothenberg; Ian D Hickson
Journal:  J Biol Chem       Date:  2013-10-09       Impact factor: 5.157

Review 8.  Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing.

Authors:  Danielle N Gallagher; James E Haber
Journal:  ACS Chem Biol       Date:  2017-11-14       Impact factor: 5.100

9.  Homologous recombination assay for interstrand cross-link repair.

Authors:  Koji Nakanishi; Francesca Cavallo; Erika Brunet; Maria Jasin
Journal:  Methods Mol Biol       Date:  2011

10.  Bisbenzamidine derivative, pentamidine represses DNA damage response through inhibition of histone H2A acetylation.

Authors:  Junya Kobayashi; Akihiro Kato; Yosuke Ota; Reiko Ohba; Kenshi Komatsu
Journal:  Mol Cancer       Date:  2010-02-09       Impact factor: 27.401
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