Literature DB >> 21660700

Homologous recombination assay for interstrand cross-link repair.

Koji Nakanishi1, Francesca Cavallo, Erika Brunet, Maria Jasin.   

Abstract

DNA interstrand cross-links (ICLs) covalently link both strands of the DNA duplex, impeding cellular processes like DNA replication. Homologous recombination (HR) is considered to be a major pathway for the repair of ICLs in mammalian cells as mutants for HR components are highly sensitive to DNA-damaging agents that cause ICLs. This chapter describes GFP assays to measure HR following site-specific ICL formation with psoralen through DNA triplex technology. This approach can be used to determine the genetic requirements for ICL-induced HR in relation to those involved in HR repair of other DNA lesions such as double-strand breaks.

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Year:  2011        PMID: 21660700      PMCID: PMC3261721          DOI: 10.1007/978-1-61779-129-1_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  23 in total

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