Literature DB >> 15466579

Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR.

David Rodríguez-Lázaro1, Anna Jofré, Teresa Aymerich, Marta Hugas, Maria Pla.   

Abstract

We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.

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Year:  2004        PMID： 15466579      PMCID： PMC522080          DOI： 10.1128/AEM.70.10.6299-6301.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

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  9 in total

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9.  Establishment of a simple and rapid identification method for Listeria spp. by using high-resolution melting analysis, and its application in food industry.

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  9 in total

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