Literature DB >> 6307828

Revised sequence of the tetracycline-resistance gene of pBR322.

K W Peden.   

Abstract

A revised sequence of the tetracycline-resistance gene of pBR322 is reported. The change, the presence of an additional CG base pair at position 526, adjusts the published sequence to allow an open reading frame from nucleotides 86-1273 (new number) and increases the size of the plasmid to 4363 bp. The predicted polypeptide encoded by this region would contain 396 amino acid residues and have a calculated Mr of 41518. A polypeptide of the predicted size has been reported previously when pBR322 is used as template in the maxicell system.

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Year:  1983        PMID： 6307828     DOI： 10.1016/0378-1119(83)90112-9

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  117 in total

1.  Nomenclature for new tetracycline resistance determinants.

Authors:  S B Levy; L M McMurry; T M Barbosa; V Burdett; P Courvalin; W Hillen; M C Roberts; J I Rood; D E Taylor
Journal:  Antimicrob Agents Chemother       Date:  1999-06       Impact factor: 5.191

2.  TnphoA and TnphoA' elements for making and switching fusions for study of transcription, translation, and cell surface localization.

Authors:  M R Wilmes-Riesenberg; B L Wanner
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

3.  Shuttle vectors conferring hygromycin B resistance to E. coli and to mammalian cells. Differential expression of carboxyterminal fusion proteins.

Authors:  F A Asselbergs; R Pronk
Journal:  Mol Biol Rep       Date:  1992-11       Impact factor: 2.316

4.  Cloning and expression of the HpaI restriction-modification genes.

Authors:  H Ito; H Shimato; A Sadaoka; H Kotani; F Kimizuka; I Kato
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971

5.  Training back-propagation neural networks to define and detect DNA-binding sites.

Authors:  M C O'Neill
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

6.  Formation of supercoiling domains in plasmid pBR322.

Authors:  J K Lodge; T Kazic; D E Berg
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

7.  The presence of the region on pBR322 that encodes resistance to tetracycline is responsible for high levels of plasmid DNA knotting in Escherichia coli DNA topoisomerase I deletion mutant.

Authors:  K Shishido; S Ishii; N Komiyama
Journal:  Nucleic Acids Res       Date:  1989-12-11       Impact factor: 16.971

8.  Structural analysis of human papillomavirus type 6c isolates from condyloma acuminatum and juvenile-onset and adult-onset laryngeal papillomata.

Authors:  L Metcalfe; S L Chen; P Mounts
Journal:  Virus Genes       Date:  1989-09       Impact factor: 2.332

9.  Tetracycline resistance element of pBR322 mediates potassium transport.

Authors:  D C Dosch; F F Salvacion; W Epstein
Journal:  J Bacteriol       Date:  1984-12       Impact factor: 3.490

10.  Functional expression of the genes of Escherichia coli in gram-positive Corynebacterium glutamicum.

Authors:  A Ozaki; R Katsumata; T Oka; A Furuya
Journal:  Mol Gen Genet       Date:  1984
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