Literature DB >> 15395569

Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.

A H COONS, M H KAPLAN.   

Abstract

Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.

Entities:  

Keywords:  ANTIGENS AND ANTIBODIES

Mesh:

Substances:

Year:  1950        PMID: 15395569      PMCID: PMC2135948          DOI: 10.1084/jem.91.1.1

Source DB:  PubMed          Journal:  J Exp Med        ISSN: 0022-1007            Impact factor:   14.307


  6 in total

1.  Phenyl isocyanate protein compounds and their immunological properties.

Authors:  S J Hopkins; A Wormall
Journal:  Biochem J       Date:  1933       Impact factor: 3.857

2.  The Zone of Localization of Anti-Mouse-Kidney Serum as Determined by Radioautographs.

Authors:  D Pressman; R F Hill; F W Foote
Journal:  Science       Date:  1949-01-21       Impact factor: 47.728

3.  The Permeability of the Renal Glomeruli of Several Mammalian Species to Labelled Proteins.

Authors:  H Smetana
Journal:  Am J Pathol       Date:  1947-03       Impact factor: 4.307

4.  Localization of antigen in tissue cells; antigens of rickettsiae and mumps virus.

Authors:  A H COONS; J C SNYDER
Journal:  J Exp Med       Date:  1950-01-01       Impact factor: 14.307

5.  Localization of antigen in tissue cells; cellular distribution of pneumococcal polysaccharides types II and III in the mouse.

Authors:  M E KAPLAN; A H COONS; H W DEANE
Journal:  J Exp Med       Date:  1950-01-01       Impact factor: 14.307

6.  THE EFFECT OF COMBINATION WITH DIAZO COMPOUNDS ON THE IMMUNOLOGICAL REACTIVITY OF ANTIBODIES.

Authors:  H Eagle; D E Smith; P Vickers
Journal:  J Exp Med       Date:  1936-04-30       Impact factor: 14.307

  6 in total
  483 in total

1.  A possible autoimmune parathyroiditis following ozone inhalation. II. A histopathologic, ultrastructural, and immunofluorescent study.

Authors:  O S Atwal; B S Samagh; M K Bhatnagar
Journal:  Am J Pathol       Date:  1975-07       Impact factor: 4.307

2.  An evaluation of the role of leukocytes in the pathogenesis of experimentally induced corneal vascularization.

Authors:  C H Fromer; G K Klintworth
Journal:  Am J Pathol       Date:  1975-06       Impact factor: 4.307

3.  Functional difference between SERCA2a and SERCA2b Ca2+ pumps and their modulation by phospholamban.

Authors:  H Verboomen; F Wuytack; H De Smedt; B Himpens; R Casteels
Journal:  Biochem J       Date:  1992-09-01       Impact factor: 3.857

4.  [Behavior of intramuscular, intravenous and oral gamma globulin].

Authors:  R MARTIN DU PAN; J J SCHEIDEGGER; P WENGER; B KOECHLI; J ROUX
Journal:  Blut       Date:  1959-04

5.  A simple method for detection of monoclonal isotypes.

Authors:  Y M Gomes; L N Regis; A B Carvalho; M Nakazawa; L Monjour; A F Furtado
Journal:  Appl Biochem Biotechnol       Date:  1992-07       Impact factor: 2.926

6.  TECHNICAL CONSIDERATIONS IN THE PREPARATION OF FLUORESCENT-ANTIBODY CONJUGATES.

Authors:  V J LEWIS; W L JONES; J B BROOKS; W B CHERRY
Journal:  Appl Microbiol       Date:  1964-07

7.  FLUORESCENT ANTIBODY TESTING WITH SPOROZOITES OF PLASMODIA.

Authors:  A CORRADETTI; F VEROLINI; A SEBASTIANI; A M PROIETTI; L AMATI
Journal:  Bull World Health Organ       Date:  1964       Impact factor: 9.408

8.  Transfer of experimental autoimmune thyroiditis by serum from thyroidectomized donors.

Authors:  R M Nakamura; W O Weigle
Journal:  J Exp Med       Date:  1969-08-01       Impact factor: 14.307

9.  Demonstration of gastrin cell autoantibodies in antral gastritis with avidin-biotin complex antibody technique.

Authors:  R M Uibo; K J Krohn
Journal:  Clin Exp Immunol       Date:  1984-11       Impact factor: 4.330

10.  Treatment of rat nephrotoxic nephritis. Use of 5-fluorouracil or methotrexate-5-fluorouracil association.

Authors:  D B de Almeida; P R Curi
Journal:  Clin Exp Immunol       Date:  1984-09       Impact factor: 4.330

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