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Literature DB >> 14201087

TECHNICAL CONSIDERATIONS IN THE PREPARATION OF FLUORESCENT-ANTIBODY CONJUGATES.

V J LEWIS, W L JONES, J B BROOKS, W B CHERRY.

Abstract

A comparison was made of (NH(4))(2)SO(4), HCl, ethodin, and ethanol for fractionation of rabbit antiserum prior to conjugation with fluorescein isothiocyanate. Fractionation with the salt was found to be the method of choice from the standpoints of simplicity and recovery of antibody effective in conjugates prepared from the fractions. Effects of pH, temperature, dye-protein ratio, and molarity and type of buffer upon conjugation were studied. These technical factors were adjusted to produce conjugates for Corynebacterium diphtheriae which possessed higher specific titers than did reagents obtained by previously employed techniques.

Entities: Chemical Species

Keywords: BLOOD PROTEIN ELECTROPHORESIS; CORYNEBACTERIUM DIPHTHERIAE; ESCHERICHIA COLI; EXPERIMENTAL LAB STUDY; FLUORESCEINS; FLUORESCENT ANTIBODY TECHNIC; IMMUNE SERUMS; KLEBSIELLA; RABBITS; SPECTROPHOTOMETRY; THIOCYANATES

Mesh：

Substances：

Year: 1964 PMID： 14201087 PMCID： PMC1058130 DOI： 10.1128/am.12.4.343-348.1964

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

10 in total

1. Fluorescent-antibody technic for serodiagnosis of Trypanosoma cruzi infection.

Authors: E H FIFE; L H MUSCHEL
Journal: Proc Soc Exp Biol Med Date: 1959-07

2. A comparison of fluorescein-labeled gamma-globulins purified by Rivanol and DEAE chromatography.

Authors: L H FROMMHAGEN; M J MARTINS
Journal: J Immunol Date: 1963-01 Impact factor: 5.422

3. The staining properties of human serum proteins conjugated with purified fluorescein isothiocyanate.

Authors: L H FROMMHAGEN; R S SPENDLOVE
Journal: J Immunol Date: 1962-07 Impact factor: 5.422

4. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application.

Authors: J D MARSHALL; W C EVELAND; C W SMITH
Journal: Proc Soc Exp Biol Med Date: 1958 Aug-Sep

5. Isothiocyanate compounds as fluorescent labeling agents for immune serum.

Authors: J L RIGGS; R J SEIWALD; J H BURCKHALTER; C M DOWNS; T G METCALF
Journal: Am J Pathol Date: 1958 Nov-Dec Impact factor: 4.307

6. Fluorescent-Antibody Identification of Group A Streptococci from Throat Swabs.

Authors: M D Moody; A C Siegel; B Pittman; C C Winter
Journal: Am J Public Health Nations Health Date: 1963-07

7. Demonstration of Escherichia coli 055 and 0111 antigens by means of hemagglutination test.

Authors: E NETER; L F BERTRAM; C E ARBESMAN
Journal: Proc Soc Exp Biol Med Date: 1952-02

8. Rapid presumptive identification of entero-pathogenic Escherichia coli in faecal smears by means of fluorescent antibody. 1. Preparation and testing of reagents.

Authors: B M THOMASON; W B CHERRY; B R DAVIS; A POMALES-LEBRON
Journal: Bull World Health Organ Date: 1961 Impact factor: 9.408

9. IDENTIFICATION OF CORYNEBACTERIUM DIPHTHERIAE WITH FLUORESCENT ANTIBACTERIAL REAGENTS.

Authors: M D MOODY; W L JONES
Journal: J Bacteriol Date: 1963-08 Impact factor: 3.490

10. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.

Authors: A H COONS; M H KAPLAN
Journal: J Exp Med Date: 1950-01-01 Impact factor: 14.307

10 in total

25 in total

9. The immune response to (3-amino, 5-succinylaminobenzoyl)-p-aminophenylarsenic acid. Immunofluorescent studies of cells making anti-arsanilic acid antibody or anti-m-aminosuccinanilic acid antibody.

Authors: D R Thursh
Journal: Immunology Date: 1970-06 Impact factor: 7.397

10. Fluorescent-antibody approach to study of rhizobia in soil.

Authors: E L Schmidt; R O Bakole; B B Bohlool
Journal: J Bacteriol Date: 1968-06 Impact factor: 3.490

TECHNICAL CONSIDERATIONS IN THE PREPARATION OF FLUORESCENT-ANTIBODY CONJUGATES.

1. Fluorescent-antibody technic for serodiagnosis of Trypanosoma cruzi infection.

2. A comparison of fluorescein-labeled gamma-globulins purified by Rivanol and DEAE chromatography.

3. The staining properties of human serum proteins conjugated with purified fluorescein isothiocyanate.

4. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application.

5. Isothiocyanate compounds as fluorescent labeling agents for immune serum.

6. Fluorescent-Antibody Identification of Group A Streptococci from Throat Swabs.

7. Demonstration of Escherichia coli 055 and 0111 antigens by means of hemagglutination test.

8. Rapid presumptive identification of entero-pathogenic Escherichia coli in faecal smears by means of fluorescent antibody. 1. Preparation and testing of reagents.

9. IDENTIFICATION OF CORYNEBACTERIUM DIPHTHERIAE WITH FLUORESCENT ANTIBACTERIAL REAGENTS.

10. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.

1. Stability of fluorescent antibody conjugates stored under various conditions.

2. Strain-specific differences in surface antigens of symbiotic algae.

3. Particulate Fractions of Neisseria gonorrhoeae.

4. Sediment distribution of methanogenic bacteria in lake erie and cleveland harbor.

5. Serological diversity within a terrestrial ammonia-oxidizing population.

6. Papain-treated globulins in specific and cross-reacting immunofluorescent staining.

7. Rapid fluorescein and protein assay method for fluorescent-antibody conjugates.

8. Reduction of nonspecific background staining in the fluorescent treponemal antibody-absorption test.

9. The immune response to (3-amino, 5-succinylaminobenzoyl)-p-aminophenylarsenic acid. Immunofluorescent studies of cells making anti-arsanilic acid antibody or anti-m-aminosuccinanilic acid antibody.

10. Fluorescent-antibody approach to study of rhizobia in soil.