Literature DB >> 1444357

A simple method for detection of monoclonal isotypes.

Y M Gomes1, L N Regis, A B Carvalho, M Nakazawa, L Monjour, A F Furtado.   

Abstract

A simple rapid method of enzyme labeled anti-isotype assay (ELIA) for detection of monoclonal isotype on hybridoma cells is proposed. This alternative method was first carried out on hybridoma cell lines 147C11 and 257C11 produced against Trypanosoma cruzi and male accessory secretion of Panstrongylus megistus, respectively. The monoclonal antibodies produced by these hybridoma were characterized by this method as IgM (147C11) and IgG1 (257C23) isotypes, allowing evaluation of isotype without having to wait until the concentration of antibody present in the supernatant itself rises. Results were confirmed by Ouchterlony immunodiffusion. The proposed method offers the advantages of a permanent rapid procedure for light microscopy.

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Year:  1992        PMID： 1444357     DOI： 10.1007/bf02950776

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  9 in total

1.  Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

2.  Diffusion-in-gel methods for immunological analysis.

Authors:  O OUCHTERLONY
Journal:  Prog Allergy       Date:  1958

3.  Monoclonal antibodies against the flagellar fraction of epimastigotes of Trypanosoma cruzi: immunoprotection against metacyclic trypomastigotes obtained by immunization of mice with an affinity-purified antigen.

Authors:  A M Ruiz; M Esteva; E Subias; M Moreno; A Rosenstein de Campanini; E Velazquez; E L Segura
Journal:  Mol Biochem Parasitol       Date:  1990-02       Impact factor: 1.759

4.  Microplate enzyme-linked immunosorbent assay for chagas' disease.

Authors:  A Voller; C Draper; D E Bidwell; A Bartlett
Journal:  Lancet       Date:  1975-02-22       Impact factor: 79.321

5.  A better cell line for making hybridomas secreting specific antibodies.

Authors:  M Shulman; C D Wilde; G Köhler
Journal:  Nature       Date:  1978-11-16       Impact factor: 49.962

6.  Cell surface antigens of Trypanosoma cruzi: use of monoclonal antibodies to identify and isolate an epimastigote specific glycoprotein.

Authors:  D Snary; M A Ferguson; M T Scott; A K Allen
Journal:  Mol Biochem Parasitol       Date:  1981-10       Impact factor: 1.759

7.  [Monoclonal antibodies against defined Leishmania antigens protect mice against infection by different species of Leishmania].

Authors:  L Monjour; A Berneman; I Vouldoukis; M Domurado; M C Guillemin; C Chopin; C Alfred; A Roseto
Journal:  C R Acad Sci III       Date:  1985

8.  Antiidiotypic antibody vaccine in murine Schistosomiasis mansoni comprising the internal image of antigen.

Authors:  T F Kresina; G R Olds
Journal:  J Clin Invest       Date:  1989-03       Impact factor: 14.808

9.  Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.

Authors:  A H COONS; M H KAPLAN
Journal:  J Exp Med       Date:  1950-01-01       Impact factor: 14.307
  9 in total
  1 in total

1.  A monoclonal antibody against blood forms of Trypanosoma cruzi lyses the parasite in vitro and inhibits host cell invasion.

Authors:  Y M Gomes; F G Abath; A F Furtado; L N Regis; M Nakasawa; L T Montenegro; I Vouldoukis; C Alfred-Morin; L Monjour
Journal:  Appl Biochem Biotechnol       Date:  1995-01       Impact factor: 2.926
  1 in total

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