Literature DB >> 15388481

Rapid detection and sequence-specific differentiation of extended-spectrum beta-lactamase GES-2 from Pseudomonas aeruginosa by use of a real-time PCR assay.

Gerhard F Weldhagen1.   

Abstract

The LightCycler was compared to nested PCR for the detection of bla(GES/IBC) genes from 100 Pseudomonas aeruginosa clinical isolates. The real-time PCR assay detected a bla(GES/IBC) gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing.

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Year:  2004        PMID： 15388481      PMCID： PMC521898          DOI： 10.1128/AAC.48.10.4059-4062.2004

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  20 in total

1.  Biochemical sequence analyses of GES-1, a novel class A extended-spectrum beta-lactamase, and the class 1 integron In52 from Klebsiella pneumoniae.

Authors:  L Poirel; I Le Thomas; T Naas; A Karim; P Nordmann
Journal:  Antimicrob Agents Chemother       Date:  2000-03       Impact factor: 5.191

2.  Use of the ligase detection reaction-polymerase chain reaction to identify point mutations in extended-spectrum beta-lactamases.

Authors:  C Niederhauser; L Kaempf; I Heinzer
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2000-06       Impact factor: 3.267

3.  Real-time PCR and melting curve analysis for reliable and rapid detection of SHV extended-spectrum beta-lactamases.

Authors:  C C Randegger; H Hächler
Journal:  Antimicrob Agents Chemother       Date:  2001-06       Impact factor: 5.191

4.  IBC-1, a novel integron-associated class A beta-lactamase with extended-spectrum properties produced by an Enterobacter cloacae clinical strain.

Authors:  P Giakkoupi; L S Tzouvelekis; A Tsakris; V Loukova; D Sofianou; E Tzelepi
Journal:  Antimicrob Agents Chemother       Date:  2000-09       Impact factor: 5.191

5.  Development of "oligotyping" for characterization and molecular epidemiology of TEM beta-lactamases in members of the family Enterobacteriaceae.

Authors:  C Mabilat; P Courvalin
Journal:  Antimicrob Agents Chemother       Date:  1990-11       Impact factor: 5.191

6.  PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain.

Authors:  F H M'Zali; J Heritage; D M Gascoyne-Binzi; A M Snelling; P M Hawkey
Journal:  J Antimicrob Chemother       Date:  1998-01       Impact factor: 5.790

7.  Rapid discriminatory detection of genes coding for SHV beta-lactamases by ligase chain reaction.

Authors:  J Kim; H J Lee
Journal:  Antimicrob Agents Chemother       Date:  2000-07       Impact factor: 5.191

8.  Genomic diversity of the genus Stenotrophomonas.

Authors:  L Hauben; L Vauterin; E R Moore; B Hoste; J Swings
Journal:  Int J Syst Bacteriol       Date:  1999-10

9.  OXA-18, a class D clavulanic acid-inhibited extended-spectrum beta-lactamase from Pseudomonas aeruginosa.

Authors:  L N Philippon; T Naas; A T Bouthors; V Barakett; P Nordmann
Journal:  Antimicrob Agents Chemother       Date:  1997-10       Impact factor: 5.191

10.  Sequence-selective recognition of extended-spectrum beta-lactamase GES-2 by a competitive, peptide nucleic acid-based multiplex PCR assay.

Authors:  Gerhard F Weldhagen
Journal:  Antimicrob Agents Chemother       Date:  2004-09       Impact factor: 5.191
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  6 in total

1.  Detection of extended-spectrum beta-lactamases in clinical isolates of Pseudomonas aeruginosa.

Authors:  Xiaofei Jiang; Zhe Zhang; Min Li; Danqiu Zhou; Feiyi Ruan; Yuan Lu
Journal:  Antimicrob Agents Chemother       Date:  2006-09       Impact factor: 5.191

2.  Pyrosequencing as a rapid tool for identification of GES-type extended-spectrum beta-lactamases.

Authors:  Laurent Poirel; Thierry Naas; Patrice Nordmann
Journal:  J Clin Microbiol       Date:  2006-08       Impact factor: 5.948

3.  BEL-2, an extended-spectrum beta-lactamase with increased activity toward expanded-spectrum cephalosporins in Pseudomonas aeruginosa.

Authors:  Laurent Poirel; Jean-Denis Docquier; Filomena De Luca; Annemie Verlinde; Louis Ide; Gian Maria Rossolini; Patrice Nordmann
Journal:  Antimicrob Agents Chemother       Date:  2009-11-02       Impact factor: 5.191

4.  Molecular analysis of population structure and antibiotic resistance of Klebsiella isolates from a three-year surveillance program in Florence hospitals, Italy.

Authors:  F Donnarumma; C Indorato; G Mastromei; E Goti; P Nicoletti; P Pecile; R Fanci; A Bosi; E Casalone
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-07-09       Impact factor: 3.267

5.  Identification of CTX-M-type extended-spectrum-beta-lactamase genes using real-time PCR and pyrosequencing.

Authors:  Thierry Naas; Cynthia Oxacelay; Patrice Nordmann
Journal:  Antimicrob Agents Chemother       Date:  2006-11-06       Impact factor: 5.191

6.  Antimicrobial Resistance Patterns and Prevalence of blaPER-1 and blaVEB-1 Genes Among ESBL-producing Pseudomonas aeruginosa Isolates in West of Iran.

Authors:  Mohammad Yousef Alikhani; Zahra Karimi Tabar; Fatemeh Mihani; Enayat Kalantar; Pegman Karami; Mahnaz Sadeghi; Shiva Ahdi Khosroshahi; Safar Farajnia
Journal:  Jundishapur J Microbiol       Date:  2014-01-01       Impact factor: 0.747
  6 in total

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