Literature DB >> 15363793

Cryo-electron microscopy of vitreous sections of native biological cells and tissues.

Ashraf Al-Amoudi1, Lars P O Norlen, Jacques Dubochet.   

Abstract

Cryo-electron microscopy of vitreous sections (CEMOVIS) is, in principle, the ultimate method of specimen preparation. It consists in ultra-rapid cooling of a sizable sample of biological material that is cut into thin sections. These are subsequently observed at low temperature in their fully hydrated vitreous state. Here, we show that CEMOVIS reveals the native state of cells and tissues with unprecedented quality and resolution. What is seen differs considerably from what conventional electron microscopy has shown previously and it is seen with more details. Our findings are demonstrated with images of cyanobacteria and skin.

Mesh：

Year:  2004        PMID： 15363793     DOI： 10.1016/j.jsb.2004.03.010

Source DB:  PubMed          Journal:  J Struct Biol        ISSN： 1047-8477            Impact factor:   2.867


  69 in total

1.  Freezing in sealed capillaries for preparation of frozen hydratedsections.

Authors:  S Yakovlev; K H Downing
Journal:  J Microsc       Date:  2011-12       Impact factor: 1.758

Review 2.  Cryo-electron microscopy of vitreous sections.

Authors:  Ashraf Al-Amoudi; Jiin-Ju Chang; Amélie Leforestier; Alasdair McDowall; Laurée Michel Salamin; Lars P O Norlén; Karsten Richter; Nathalie Sartori Blanc; Daniel Studer; Jacques Dubochet
Journal:  EMBO J       Date:  2004-08-19       Impact factor: 11.598

3.  Lamellar body ultrastructure revisited: high-pressure freezing and cryo-electron microscopy of vitreous sections.

Authors:  Dimitri Vanhecke; Gudrun Herrmann; Werner Graber; Therese Hillmann-Marti; Christian Mühlfeld; Daniel Studer; Matthias Ochs
Journal:  Histochem Cell Biol       Date:  2010-09-01       Impact factor: 4.304

4.  Membrane-impermeable cross-linking provides evidence for homophilic, isoform-specific binding of desmosomal cadherins in epithelial cells.

Authors:  Zhuxiang Nie; Anita Merritt; Mansour Rouhi-Parkouhi; Lydia Tabernero; David Garrod
Journal:  J Biol Chem       Date:  2010-11-22       Impact factor: 5.157

Review 5.  Minicells, Back in Fashion.

Authors:  Madeline M Farley; Bo Hu; William Margolin; Jun Liu
Journal:  J Bacteriol       Date:  2016-03-31       Impact factor: 3.490

6.  The mammalian central nervous synaptic cleft contains a high density of periodically organized complexes.

Authors:  Benoît Zuber; Irina Nikonenko; Paul Klauser; Dominique Muller; Jacques Dubochet
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-14       Impact factor: 11.205

7.  Chromatin organization and radio resistance in the bacterium Gemmata obscuriglobus.

Authors:  Arnon Lieber; Andrew Leis; Ariel Kushmaro; Abraham Minsky; Ohad Medalia
Journal:  J Bacteriol       Date:  2008-12-12       Impact factor: 3.490

8.  Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography.

Authors:  Chyongere Hsieh; Thomas Schmelzer; Gregory Kishchenko; Terence Wagenknecht; Michael Marko
Journal:  J Struct Biol       Date:  2013-11-06       Impact factor: 2.867

Review 9.  Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell.

Authors:  Axel A Ekman; Jian-Hua Chen; Jessica Guo; Gerry McDermott; Mark A Le Gros; Carolyn A Larabell
Journal:  Biol Cell       Date:  2016-11-14       Impact factor: 4.458

10.  Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells.

Authors:  Ai Leen Koh; Catherine M Shachaf; Sailaja Elchuri; Garry P Nolan; Robert Sinclair
Journal:  Ultramicroscopy       Date:  2008-10-02       Impact factor: 2.689
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.