Literature DB >> 15080697

Line narrowing in methyl-TROSY using zero-quantum 1H-13C NMR spectroscopy.

Vitali Tugarinov1, Remco Sprangers, Lewis E Kay.   

Abstract

An enhanced sensitivity zero-quantum correlation experiment is proposed for recording (1)H-(13)C correlations of methyl groups in highly deuterated, methyl protonated large proteins. The zero-quantum spectra benefit from TROSY-effects in which both intra- and inter-methyl dipolar relaxation interactions are minimized. Applications to malate synthase G at 5 degrees C (81 kDa single polypeptide chain enzyme, correlation time of 118 ns) and lysine decarboxylase at 45 degrees C (810 kDa decameric enzyme) are presented showing significant improvements in resolution relative to corresponding HMQC data sets, with only slight decreases (approximately 10%) in sensitivity.

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Year:  2004        PMID： 15080697     DOI： 10.1021/ja039732s

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  25 in total

1.  Estimating side-chain order in methyl-protonated, perdeuterated proteins via multiple-quantum relaxation violated coherence transfer NMR spectroscopy.

Authors:  Hechao Sun; Raquel Godoy-Ruiz; Vitali Tugarinov
Journal:  J Biomol NMR       Date:  2012-03       Impact factor: 2.835

2.  The crystal structure of a Munc13 C-terminal module exhibits a remarkable similarity to vesicle tethering factors.

Authors:  Wei Li; Cong Ma; Rong Guan; Yibin Xu; Diana R Tomchick; Josep Rizo
Journal:  Structure       Date:  2011-10-12       Impact factor: 5.006

3.  Proton-evolved local-field solid-state NMR studies of cytochrome b5 embedded in bicelles, revealing both structural and dynamical information.

Authors:  Ronald Soong; Pieter E S Smith; Jiadi Xu; Kazutoshi Yamamoto; Sang-Choul Im; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  J Am Chem Soc       Date:  2010-04-28       Impact factor: 15.419

4.  Direct methods and residue type specific isotope labeling in NMR structure determination and model-driven sequential assignment.

Authors:  Andreas Schedlbauer; Renate Auer; Karin Ledolter; Martin Tollinger; Karin Kloiber; Roman Lichtenecker; Simon Ruedisser; Ulrich Hommel; Walther Schmid; Robert Konrat; Georg Kontaxis
Journal:  J Biomol NMR       Date:  2008-09-02       Impact factor: 2.835

5.  CaBP1, a neuronal Ca2+ sensor protein, inhibits inositol trisphosphate receptors by clamping intersubunit interactions.

Authors:  Congmin Li; Masahiro Enomoto; Ana M Rossi; Min-Duk Seo; Taufiq Rahman; Peter B Stathopulos; Colin W Taylor; Mitsuhiko Ikura; James B Ames
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-06       Impact factor: 11.205

6.  Detection of chemical exchange in methyl groups of macromolecules.

Authors:  Michelle L Gill; Andrew Hsu; Arthur G Palmer
Journal:  J Biomol NMR       Date:  2019-08-12       Impact factor: 2.835

7.  Multiplet-filtered and gradient-selected zero-quantum TROSY experiments for 13C1H3 methyl groups in proteins.

Authors:  Michelle L Gill; Arthur G Palmer
Journal:  J Biomol NMR       Date:  2011-09-15       Impact factor: 2.835

8.  Towards unambiguous assignment of methyl-containing residues by double and triple sensitivity-enhanced HCCmHm-TOCSY experiments.

Authors:  Peter Würtz; Maarit Hellman; Helena Tossavainen; Perttu Permi
Journal:  J Biomol NMR       Date:  2006-09-09       Impact factor: 2.835

9.  Probing microsecond time scale dynamics in proteins by methyl (1)H Carr-Purcell-Meiboom-Gill relaxation dispersion NMR measurements. Application to activation of the signaling protein NtrC(r).

Authors:  Renee Otten; Janice Villali; Dorothee Kern; Frans A A Mulder
Journal:  J Am Chem Soc       Date:  2010-11-08       Impact factor: 15.419

Review 10.  Methyl-Based NMR Spectroscopy Methods for Uncovering Structural Dynamics in Large Proteins and Protein Complexes.

Authors:  Zachary K Boswell; Michael P Latham
Journal:  Biochemistry       Date:  2018-10-26       Impact factor: 3.162
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