BACKGROUND: Reliable chemosensitivity testing of head and neck squamous cell carcinoma (HNSCC) still faces methodical limitations. Since stromal cell contamination has been found to preclude reliable radiosensitivity testing of HNSCC as well as chemosensitivity testing of lung tumors, the present study investigates the impact of stromal cell contamination on chemosensitivity testing of HNSCC. PATIENTS AND METHODS: Seventeen biopsies from HNSCC were analyzed. The specimens were investigated using an ex vivo colony formation assay which allows for the quantitative and separate determination of the overall, as well as the epithelial, and stromal response to carboplatin, 5-fluorouracil and docetaxel. RESULTS: The overall chemoresponse was dominated by stromal cell multidrug resistance. However, by selective evaluation of the epithelial chemoresponse, individual chemosensitivity patterns could be identified. CONCLUSION: Multidrug-resistant stromal cells preclude the reliable assessment of the chemoresponse of HNSCC specimens. Carefiul correction for stromal cell effects is a prerequisite for the generation of therapeutically useful information.
BACKGROUND: Reliable chemosensitivity testing of head and neck squamous cell carcinoma (HNSCC) still faces methodical limitations. Since stromal cell contamination has been found to preclude reliable radiosensitivity testing of HNSCC as well as chemosensitivity testing of lung tumors, the present study investigates the impact of stromal cell contamination on chemosensitivity testing of HNSCC. PATIENTS AND METHODS: Seventeen biopsies from HNSCC were analyzed. The specimens were investigated using an ex vivo colony formation assay which allows for the quantitative and separate determination of the overall, as well as the epithelial, and stromal response to carboplatin, 5-fluorouracil and docetaxel. RESULTS: The overall chemoresponse was dominated by stromal cell multidrug resistance. However, by selective evaluation of the epithelial chemoresponse, individual chemosensitivity patterns could be identified. CONCLUSION: Multidrug-resistant stromal cells preclude the reliable assessment of the chemoresponse of HNSCC specimens. Carefiul correction for stromal cell effects is a prerequisite for the generation of therapeutically useful information.
Authors: Andreas Dietz; Andreas Boehm; Iris-Susanne Horn; Pierre Kruber; Ingo Bechmann; Wojciech Golusinski; Dietger Niederwieser; Ralph Dollner; Torsten W Remmerbach; Christian Wittekind; Stephan Dietzsch; Guido Hildebrandt; Gunnar Wichmann Journal: Eur Arch Otorhinolaryngol Date: 2010-01-06 Impact factor: 2.503
Authors: H J Ren; C L Zhang; R D Liu; N Li; X G Li; H K Xue; Y Guo; Z Q Wang; J Cui; L Ming Journal: Braz J Med Biol Res Date: 2017-04-13 Impact factor: 2.590
Authors: Candace M Howard; Stephen Bush; Nadim Bou Zgheib; Seth T Lirette; Antonio Cortese; Antonio Mollo; Jagan Valluri; Pier Paolo Claudio Journal: HSOA J Stem Cells Res Dev Ther Date: 2021-09-09
Authors: Amy J C Dohmen; Justin E Swartz; Michiel W M Van Den Brekel; Stefan M Willems; René Spijker; Jacques Neefjes; Charlotte L Zuur Journal: Cancers (Basel) Date: 2015-08-28 Impact factor: 6.639
Authors: M M Gerlach; F Merz; G Wichmann; C Kubick; C Wittekind; F Lordick; A Dietz; I Bechmann Journal: Br J Cancer Date: 2013-11-21 Impact factor: 7.640
Authors: Amy J C Dohmen; Joyce Sanders; Sander Canisius; Ekaterina S Jordanova; Else A Aalbersberg; Michiel W M van den Brekel; Jacques Neefjes; Charlotte L Zuur Journal: Oncotarget Date: 2018-05-18