Literature DB >> 1500529

Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction.

M Altamirano1, M T Kelly, A Wong, E T Bessuille, W A Black, J A Smith.   

Abstract

We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.

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Year:  1992        PMID： 1500529      PMCID： PMC265465          DOI： 10.1128/jcm.30.8.2173-2176.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Rapid diagnosis of tuberculous meningitis by polymerase chain reaction (PCR).

Authors:  K Kaneko; O Onodera; T Miyatake; S Tsuji
Journal:  Neurology       Date:  1990-10       Impact factor: 9.910

2.  Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis.

Authors:  K D Eisenach; M D Cave; J H Bates; J T Crawford
Journal:  J Infect Dis       Date:  1990-05       Impact factor: 5.226

3.  Use of Gen-Probe and Bactec for rapid isolation and identification of mycobacteria. Correlation of probe results with growth index.

Authors:  B A Body; N G Warren; A Spicer; D Henderson; M Chery
Journal:  Am J Clin Pathol       Date:  1990-03       Impact factor: 2.493

4.  Improved microfluorometric DNA determination in biological material using 33258 Hoechst.

Authors:  C F Cesarone; C Bolognesi; L Santi
Journal:  Anal Biochem       Date:  1979-11-15       Impact factor: 3.365

5.  Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples.

Authors:  A Brisson-Noël; B Gicquel; D Lecossier; V Lévy-Frébault; X Nassif; A J Hance
Journal:  Lancet       Date:  1989-11-04       Impact factor: 79.321

6.  Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification.

Authors:  D De Wit; L Steyn; S Shoemaker; M Sogin
Journal:  J Clin Microbiol       Date:  1990-11       Impact factor: 5.948

7.  A DNA primer/probe system for the rapid and sensitive detection of Mycobacterium tuberculosis-complex pathogens.

Authors:  N T Ralphs; S Garrett; R Morse; J B Cookson; P W Andrew; G J Boulnois
Journal:  J Appl Bacteriol       Date:  1991-03

8.  DNA probes for mycobacteria. I. Isolation of DNA probes for the identification of Mycobacterium tuberculosis complex and for mycobacteria other than tuberculosis (MOTT).

Authors:  R N Picken; S J Plotch; Z Wang; B C Lin; J J Donegan; H L Yang
Journal:  Mol Cell Probes       Date:  1988-06       Impact factor: 2.365

9.  The detection of mycobacterial DNA sequences in uncultured clinical specimens with cloned Mycobacterium tuberculosis DNA as probes.

Authors:  C C Pao; S S Lin; S Y Wu; W M Juang; C H Chang; J Y Lin
Journal:  Tubercle       Date:  1988-03

10.  Improved detection times for Mycobacterium avium complex and Mycobacterium tuberculosis with the BACTEC radiometric system.

Authors:  J M Kirihara; S L Hillier; M B Coyle
Journal:  J Clin Microbiol       Date:  1985-11       Impact factor: 5.948
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  11 in total

1.  Evaluation of PCR using TRC(4) and IS6110 primers in detection of tuberculous meningitis.

Authors:  S Narayanan; V Parandaman; P R Narayanan; P Venkatesan; C Girish; S Mahadevan; S Rajajee
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

2.  Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples.

Authors:  M Maher; M Glennon; G Martinazzo; E Turchetti; S Marcolini; T Smith; M T Dawson
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

3.  Diagnosis of cutaneous tuberculosis in biopsy specimens by PCR and southern blotting.

Authors:  E Quirós; M C Maroto; A Bettinardi; I González; G Piédrola
Journal:  J Clin Pathol       Date:  1996-11       Impact factor: 3.411

4.  Detection of Mycobacterium tuberculosis in clinical specimens by polymerase chain reaction and Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test.

Authors:  C Abe; K Hirano; M Wada; Y Kazumi; M Takahashi; Y Fukasawa; T Yoshimura; C Miyagi; S Goto
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

Review 5.  Diagnosis of tuberculosis: available technologies, limitations, and possibilities.

Authors:  Sanjay K Garg; R P Tiwari; Dileep Tiwari; Rupinder Singh; Dolly Malhotra; V K Ramnani; G B K S Prasad; Ramesh Chandra; M Fraziano; V Colizzi; Prakash S Bisen
Journal:  J Clin Lab Anal       Date:  2003       Impact factor: 2.352

6.  Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens.

Authors:  K G Beavis; M B Lichty; D L Jungkind; O Giger
Journal:  J Clin Microbiol       Date:  1995-10       Impact factor: 5.948

Review 7.  Tuberculosis in the 1990s. Issues for primary care physicians.

Authors:  J M Fitzgerald
Journal:  Can Fam Physician       Date:  1995-06       Impact factor: 3.275

8.  Standardization of a TaqMan-based real-time PCR for the detection of Mycobacterium tuberculosis-complex in human sputum.

Authors:  Francesca Barletta; Koen Vandelannoote; Jimena Collantes; Carlton A Evans; Jorge Arévalo; Leen Rigouts
Journal:  Am J Trop Med Hyg       Date:  2014-08-11       Impact factor: 2.345

9.  In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression.

Authors:  Laura L Flores; Madhukar Pai; John M Colford; Lee W Riley
Journal:  BMC Microbiol       Date:  2005-10-03       Impact factor: 3.605

10.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1992-11-11       Impact factor: 16.971
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