Literature DB >> 8862607

Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples.

M Maher1, M Glennon, G Martinazzo, E Turchetti, S Marcolini, T Smith, M T Dawson.   

Abstract

We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay.

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Year:  1996        PMID： 8862607      PMCID： PMC229240          DOI： 10.1128/jcm.34.9.2307-2308.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

2.  Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.

Authors:  Y Miyazaki; H Koga; S Kohno; M Kaku
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

3.  Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification.

Authors:  D De Wit; L Steyn; S Shoemaker; M Sogin
Journal:  J Clin Microbiol       Date:  1990-11       Impact factor: 5.948

4.  Detection and identification of Mycobacterium tuberculosis by DNA amplification.

Authors:  C C Pao; T S Yen; J B You; J S Maa; E H Fiss; C H Chang
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

5.  A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples.

Authors:  L F Kox; D Rhienthong; A M Miranda; N Udomsantisuk; K Ellis; J van Leeuwen; S van Heusden; S Kuijper; A H Kolk
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948

6.  Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA.

Authors:  V Jonas; M J Alden; J I Curry; K Kamisango; C A Knott; R Lankford; J M Wolfe; D F Moore
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

7.  Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system.

Authors:  A H Kolk; A R Schuitema; S Kuijper; J van Leeuwen; P W Hermans; J D van Embden; R A Hartskeerl
Journal:  J Clin Microbiol       Date:  1992-10       Impact factor: 5.948

8.  Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory.

Authors:  J E Clarridge; R M Shawar; T M Shinnick; B B Plikaytis
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

9.  Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR.

Authors:  D F Moore; J I Curry
Journal:  J Clin Microbiol       Date:  1995-10       Impact factor: 5.948

10.  Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction.

Authors:  M Altamirano; M T Kelly; A Wong; E T Bessuille; W A Black; J A Smith
Journal:  J Clin Microbiol       Date:  1992-08       Impact factor: 5.948
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  4 in total

1.  A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens.

Authors:  A M Kearns; R Freeman; M Steward; J G Magee
Journal:  J Clin Pathol       Date:  1998-12       Impact factor: 3.411

2.  Transport of digested decontaminated sputum specimens to a central laboratory for testing for M. tuberculosis by Amplicor MTB test.

Authors:  U Ni Riain; M Cormican; J Flynn
Journal:  Ir J Med Sci       Date:  1998 Apr-Jun       Impact factor: 1.568

3.  Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.

Authors:  P Morata; M I Queipo-Ortuño; J de Dios Colmenero
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

4.  In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression.

Authors:  Laura L Flores; Madhukar Pai; John M Colford; Lee W Riley
Journal:  BMC Microbiol       Date:  2005-10-03       Impact factor: 3.605
  4 in total

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