Literature DB >> 1476716

DNase I treatment of Taq DNA polymerase for complete PCR decontamination.

P A Rochelle1, A J Weightman, J C Fry.   

Abstract

Mesh：

Substances：

Year:  1992        PMID： 1476716

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


× No keyword cloud information.
  11 in total

1.  Contamination and sensitivity issues with a real-time universal 16S rRNA PCR.

Authors:  C E Corless; M Guiver; R Borrow; V Edwards-Jones; E B Kaczmarski; A J Fox
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

2.  DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.

Authors:  Alexandra Heininger; Marlies Binder; Andreas Ellinger; Konrad Botzenhart; Klaus Unertl; Gerd Döring
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

3.  Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting.

Authors:  Tim Schuurman; Richard F de Boer; Anna M D Kooistra-Smid; Anton A van Zwet
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

4.  Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR.

Authors:  Sven Klaschik; Lutz E Lehmann; Ansgar Raadts; Andreas Hoeft; Frank Stuber
Journal:  Mol Biotechnol       Date:  2002-11       Impact factor: 2.695

5.  Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.

Authors:  Tamara Newsome; Bing-Jie Li; Nianxiang Zou; Shyh-Ching Lo
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

Review 6.  False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy.

Authors:  A Borst; A T A Box; A C Fluit
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-03-10       Impact factor: 3.267

7.  A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination.

Authors:  Hideki Niimi; Masashi Mori; Homare Tabata; Hiroshi Minami; Tomohiro Ueno; Shirou Hayashi; Isao Kitajima
Journal:  J Clin Microbiol       Date:  2011-07-20       Impact factor: 5.948

8.  Transformation of Pythium aphanidermatum to geneticin resistance.

Authors:  John J Weiland
Journal:  Curr Genet       Date:  2003-02-07       Impact factor: 3.886

9.  An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications.

Authors:  Sophie Champlot; Camille Berthelot; Mélanie Pruvost; E Andrew Bennett; Thierry Grange; Eva-Maria Geigl
Journal:  PLoS One       Date:  2010-09-28       Impact factor: 3.240

10.  Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria.

Authors:  Rudolph Spangler; Noel L Goddard; David S Thaler
Journal:  PLoS One       Date:  2009-09-15       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.