Literature DB >> 21775543

A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination.

Hideki Niimi1, Masashi Mori, Homare Tabata, Hiroshi Minami, Tomohiro Ueno, Shirou Hayashi, Isao Kitajima.   

Abstract

To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.

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Year:  2011        PMID: 21775543      PMCID: PMC3165589          DOI: 10.1128/JCM.00584-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  26 in total

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Journal:  Mol Genet Metab       Date:  1999-03       Impact factor: 4.797

2.  Fluorescence-based DNA polymerase assay.

Authors:  H Tveit; T Kristensen
Journal:  Anal Biochem       Date:  2001-02-01       Impact factor: 3.365

3.  Optimization of real-time PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates.

Authors:  Tamimount Mohammadi; Henk W Reesink; Christina M J E Vandenbroucke-Grauls; Paul H M Savelkoul
Journal:  J Clin Microbiol       Date:  2003-10       Impact factor: 5.948

4.  Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.

Authors:  Sebastian Philipp; Hartwig P Huemer; Eveline U Irschick; Christoph Gassner
Journal:  Transfus Med Hemother       Date:  2010-01-07       Impact factor: 3.747

5.  Taq polymerase contains bacterial DNA of unknown origin.

Authors:  K H Rand; H Houck
Journal:  Mol Cell Probes       Date:  1990-12       Impact factor: 2.365

6.  Inducible virus-mediated expression of a foreign protein in suspension-cultured plant cells.

Authors:  K Dohi; M Nishikiori; A Tamai; M Ishikawa; T Meshi; M Mori
Journal:  Arch Virol       Date:  2006-01-19       Impact factor: 2.574

Review 7.  Bacterial evolution.

Authors:  C R Woese
Journal:  Microbiol Rev       Date:  1987-06

8.  Identification and elimination of DNA sequences in Taq DNA polymerase.

Authors:  M S Hughes; L A Beck; R A Skuce
Journal:  J Clin Microbiol       Date:  1994-08       Impact factor: 5.948

9.  Removal of contaminating DNA from polymerase chain reaction using ethidium monoazide.

Authors:  Andreas Rueckert; Hugh W Morgan
Journal:  J Microbiol Methods       Date:  2006-12-20       Impact factor: 2.363

10.  Frequency of isolation of pathogens from bloodstream, nosocomial pneumonia, skin and soft tissue, and urinary tract infections occurring in European patients.

Authors:  A C Fluit; F J Schmitz; J Verhoef
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2001-03       Impact factor: 3.267

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  8 in total

1.  Construction, Expression, and Characterization of Recombinant Pfu DNA Polymerase in Escherichia coli.

Authors:  Wenjun Zheng; Qingsong Wang; Qun Bi
Journal:  Protein J       Date:  2016-04       Impact factor: 2.371

2.  Characterization of Recombinant Thermococcus kodakaraensis (KOD) DNA Polymerases Produced Using Silkworm-Baculovirus Expression Vector System.

Authors:  Mami Yamashita; Jian Xu; Daisuke Morokuma; Kazuma Hirata; Masato Hino; Hiroaki Mon; Masateru Takahashi; Samir M Hamdan; Kosuke Sakashita; Kazuhiro Iiyama; Yutaka Banno; Takahiro Kusakabe; Jae Man Lee
Journal:  Mol Biotechnol       Date:  2017-06       Impact factor: 2.695

3.  Improved efficiency and robustness in qPCR and multiplex end-point PCR by twisted intercalating nucleic acid modified primers.

Authors:  Uffe Vest Schneider; Nikolaj Dam Mikkelsen; Anja Lindqvist; Limei Meng Okkels; Nina Jøhnk; Gorm Lisby
Journal:  PLoS One       Date:  2012-06-06       Impact factor: 3.240

4.  Comparison of DNA extraction methods for microbial community profiling with an application to pediatric bronchoalveolar lavage samples.

Authors:  Dana Willner; Joshua Daly; David Whiley; Keith Grimwood; Claire E Wainwright; Philip Hugenholtz
Journal:  PLoS One       Date:  2012-04-13       Impact factor: 3.240

5.  Eukaryote-Made Thermostable DNA Polymerase Enables Rapid PCR-Based Detection of Mycoplasma, Ureaplasma and Other Bacteria in the Amniotic Fluid of Preterm Labor Cases.

Authors:  Tomohiro Ueno; Hideki Niimi; Noriko Yoneda; Satoshi Yoneda; Masashi Mori; Homare Tabata; Hiroshi Minami; Shigeru Saito; Isao Kitajima
Journal:  PLoS One       Date:  2015-06-04       Impact factor: 3.240

6.  Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose.

Authors:  Hirokazu Takahashi; Hiroyuki Yamazaki; Satoshi Akanuma; Hiroko Kanahara; Toshiyuki Saito; Tomoyuki Chimuro; Takayoshi Kobayashi; Toshio Ohtani; Kimiko Yamamoto; Shigeru Sugiyama; Toshiro Kobori
Journal:  PLoS One       Date:  2014-02-05       Impact factor: 3.240

7.  Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media.

Authors:  Saara Sillanpää; Lenka Kramna; Sami Oikarinen; Markku Sipilä; Markus Rautiainen; Janne Aittoniemi; Jussi Laranne; Heikki Hyöty; Ondrej Cinek
Journal:  mSphere       Date:  2017-03-22       Impact factor: 4.389

8.  Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection.

Authors:  Hideki Niimi; Tomohiro Ueno; Shirou Hayashi; Akihito Abe; Takahiro Tsurue; Masashi Mori; Homare Tabata; Hiroshi Minami; Michihiko Goto; Makoto Akiyama; Yoshihiro Yamamoto; Shigeru Saito; Isao Kitajima
Journal:  Sci Rep       Date:  2015-07-28       Impact factor: 4.379

  8 in total

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