| Literature DB >> 21775543 |
Hideki Niimi1, Masashi Mori, Homare Tabata, Hiroshi Minami, Tomohiro Ueno, Shirou Hayashi, Isao Kitajima.
Abstract
To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.Mesh:
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Year: 2011 PMID: 21775543 PMCID: PMC3165589 DOI: 10.1128/JCM.00584-11
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948