Literature DB >> 12682181

DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.

Alexandra Heininger1, Marlies Binder, Andreas Ellinger, Konrad Botzenhart, Klaus Unertl, Gerd Döring.   

Abstract

DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerase. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used.

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Year:  2003        PMID： 12682181      PMCID： PMC153871          DOI： 10.1128/JCM.41.4.1763-1765.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  28 in total

1.  A simple method for elimination of unspecific amplifications in polymerase chain reaction.

Authors:  J K Sharma; V Gopalkrishna; B C Das
Journal:  Nucleic Acids Res       Date:  1992-11-25       Impact factor: 16.971

2.  DNase I treatment of Taq DNA polymerase for complete PCR decontamination.

Authors:  P A Rochelle; A J Weightman; J C Fry
Journal:  Biotechniques       Date:  1992-10       Impact factor: 1.993

3.  More light on PCR contamination.

Authors:  G Sarkar; S Sommer
Journal:  Nature       Date:  1990-09-27       Impact factor: 49.962

4.  Taq polymerase contains bacterial DNA of unknown origin.

Authors:  K H Rand; H Houck
Journal:  Mol Cell Probes       Date:  1990-12       Impact factor: 2.365

5.  Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction.

Authors:  K H Wilson; R B Blitchington; R C Greene
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

6.  Detection of DNA contamination in Taq polymerase.

Authors:  T M Schmidt; B Pace; N R Pace
Journal:  Biotechniques       Date:  1991-08       Impact factor: 1.993

7.  Identification and elimination of DNA sequences in Taq DNA polymerase.

Authors:  M S Hughes; L A Beck; R A Skuce
Journal:  J Clin Microbiol       Date:  1994-08       Impact factor: 5.948

8.  Decontamination of polymerase chain reaction reagents for detection of low concentrations of 16S rRNA genes.

Authors:  F Hilali; P Saulnier; E Chachaty; A Andremont
Journal:  Mol Biotechnol       Date:  1997-06       Impact factor: 2.695

9.  Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.

Authors:  A Meier; D H Persing; M Finken; E C Böttger
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

10.  The use of polymerase chain reaction to detect septicemia in critically ill patients.

Authors:  R T Cursons; E Jeyerajah; J W Sleigh
Journal:  Crit Care Med       Date:  1999-05       Impact factor: 7.598
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  15 in total

1.  Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting.

Authors:  Tim Schuurman; Richard F de Boer; Anna M D Kooistra-Smid; Anton A van Zwet
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

2.  Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification.

Authors:  Franziska Zucol; Roland A Ammann; Christoph Berger; Christoph Aebi; Martin Altwegg; Felix K Niggli; David Nadal
Journal:  J Clin Microbiol       Date:  2006-08       Impact factor: 5.948

3.  Non-culture-based methods to diagnose bloodstream infection: Does it work?

Authors:  Miha Skvarc; David Stubljar; Petra Rogina; Achim J Kaasch
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2013-06-05

4.  Expression of CD64 on neutrophils can be used to predict the severity of bloodstream infection before broad range 16S rRNA PCR.

Authors:  David Stubljar; Miha Skvarc
Journal:  Folia Microbiol (Praha)       Date:  2014-09-26       Impact factor: 2.099

5.  Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers.

Authors:  Charlotte E Davies; Katja E Hill; Melanie J Wilson; Phil Stephens; C Michael Hill; Keith G Harding; David W Thomas
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

6.  Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis.

Authors:  Nele Wellinghausen; Anna-Julia Kochem; Claudia Disqué; Helge Mühl; Susanne Gebert; Juliane Winter; Jens Matten; Samir G Sakka
Journal:  J Clin Microbiol       Date:  2009-07-01       Impact factor: 5.948

7.  Gram stain-specific-probe-based real-time PCR for diagnosis and discrimination of bacterial neonatal sepsis.

Authors:  Yi-Dong Wu; Li-Hua Chen; Xiu-Jing Wu; Shi-Qiang Shang; Jin-Tu Lou; Li-Zhong Du; Zheng-Yan Zhao
Journal:  J Clin Microbiol       Date:  2008-06-11       Impact factor: 5.948

8.  Removal of Contaminant DNA by Combined UV-EMA Treatment Allows Low Copy Number Detection of Clinically Relevant Bacteria Using Pan-Bacterial Real-Time PCR.

Authors:  Bruce Humphrey; Neil McLeod; Carrie Turner; J Mark Sutton; Paul M Dark; Geoffrey Warhurst
Journal:  PLoS One       Date:  2015-07-14       Impact factor: 3.240

9.  Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria.

Authors:  Rudolph Spangler; Noel L Goddard; David S Thaler
Journal:  PLoS One       Date:  2009-09-15       Impact factor: 3.240

10.  Use of staged molecular analysis to determine causes of unexplained central nervous system infections.

Authors:  Chien-Chin Hsu; Rafal Tokarz; Thomas Briese; Hung-Chin Tsai; Phenix-Lan Quan; W Ian Lipkin
Journal:  Emerg Infect Dis       Date:  2013       Impact factor: 6.883
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