Literature DB >> 14646130

Crystallization of a large single crystal of cubic insulin for neutron protein crystallography.

M Maeda1, T Chatake, I Tanaka, A Ostermann, N Niimura.   

Abstract

The growth of a large single crystal of cubic porcine insulin for characterization of hydrogen and hydration in cubic insulin crystals by neutron diffraction analysis is reported. Growth in D2O was investigated based on the phase diagram for cubic insulin to determine appropriate growth conditions, and a large single crystal was then successfully grown by a dialysis method to a size of 4.0 x 4.0 x 1.3 mm3. Neutron diffraction analysis of the cubic insulin crystals was carried out using a single-crystal diffractometer at the JRR-3M reactor of the Japan Atomic Energy Research Institute. In preliminary analysis, Npi appears to be protonated and Ntau deprotonated in His5 in the B-chain, whereas both Npi and Ntau are protonated in His10.

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Year:  2003        PMID： 14646130     DOI： 10.1107/s0909049503023859

Source DB:  PubMed          Journal:  J Synchrotron Radiat        ISSN： 0909-0495            Impact factor:   2.616


  9 in total

Review 1.  Neutron Laue macromolecular crystallography.

Authors:  Flora Meilleur; Dean A A Myles; Matthew P Blakeley
Journal:  Eur Biophys J       Date:  2006-08-03       Impact factor: 1.733

2.  A preliminary neutron diffraction study of rasburicase, a recombinant urate oxidase enzyme, complexed with 8-azaxanthin.

Authors:  Monika Budayova-Spano; Françoise Bonneté; Natalie Ferté; Mohamed El Hajji; Flora Meilleur; Matthew Paul Blakeley; Bertrand Castro
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-02-28

Review 3.  Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase.

Authors:  E Oksanen; M P Blakeley; F Bonneté; M T Dauvergne; F Dauvergne; M Budayova-Spano
Journal:  J R Soc Interface       Date:  2009-07-08       Impact factor: 4.118

4.  Neutron diffraction studies of Escherichia coli dihydrofolate reductase complexed with methotrexate.

Authors:  Brad Bennett; Paul Langan; Leighton Coates; Marat Mustyakimov; Benno Schoenborn; Elizabeth E Howell; Chris Dealwis
Journal:  Proc Natl Acad Sci U S A       Date:  2006-11-27       Impact factor: 11.205

5.  A neutron crystallographic analysis of T6 porcine insulin at 2.1 A resolution.

Authors:  Wakari Iwai; Taro Yamada; Kazuo Kurihara; Yuki Ohnishi; Yoichiro Kobayashi; Ichiro Tanaka; Haruyuki Takahashi; Ryota Kuroki; Taro Tamada; Nobuo Niimura
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2009-09-16

Review 6.  Neutron crystallography: opportunities, challenges, and limitations.

Authors:  Matthew P Blakeley; Paul Langan; Nobuo Niimura; Alberto Podjarny
Journal:  Curr Opin Struct Biol       Date:  2008-08-07       Impact factor: 6.809

7.  Complicated water orientations in the minor groove of the B-DNA decamer d(CCATTAATGG)2 observed by neutron diffraction measurements.

Authors:  Shigeki Arai; Toshiyuki Chatake; Takashi Ohhara; Kazuo Kurihara; Ichiro Tanaka; Nobuhiro Suzuki; Zui Fujimoto; Hiroshi Mizuno; Nobuo Niimura
Journal:  Nucleic Acids Res       Date:  2005-05-24       Impact factor: 16.971

8.  Semi-empirical model to estimate ideal conditions for the growth of large protein crystals.

Authors:  Hirohiko Nakamura; Sachiko Takahashi; Koji Inaka; Hiroaki Tanaka
Journal:  Acta Crystallogr D Struct Biol       Date:  2020-11-26       Impact factor: 7.652

9.  Determination of Histidine Protonation States in Proteins by Fast Magic Angle Spinning NMR.

Authors:  Roman Zadorozhnyi; Sucharita Sarkar; Caitlin M Quinn; Kaneil K Zadrozny; Barbie K Ganser-Pornillos; Owen Pornillos; Angela M Gronenborn; Tatyana Polenova
Journal:  Front Mol Biosci       Date:  2021-12-10
  9 in total

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