Literature DB >> 1459424

Uniparental cytogamy: a novel method for bringing micronuclear mutations of Tetrahymena into homozygous macronuclear expression with precocious sexual maturity.

E S Cole1, P J Bruns.   

Abstract

A new method of inducing self-fertilization, uniparental cytogamy, yields homozygous germinal and somatic genotypes in the ciliate Tetrahymena thermophila. Progeny are highly fertile and show a marked tendency for precocious sexual maturity. This method is highly effective in protocols designed to generate and express nonlethal dominant or recessive mutations.

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Year:  1992        PMID： 1459424      PMCID： PMC1205224     

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  21 in total

1.  Macronuclear differentiation in conjugating pairs of Tetrahymena treated with the antitubulin drug nocodazole.

Authors:  A Kaczanowski; M Ramel; J Kaczanowska; D Wheatley
Journal:  Exp Cell Res       Date:  1991-08       Impact factor: 3.905

2.  Traumatic induction of early maturity in tetrahymena.

Authors:  D L Nanney; E B Meyer
Journal:  Genetics       Date:  1977-05       Impact factor: 4.562

3.  Mass isolation and fertility testing of temperature-sensitive mutants in Tetrahymena.

Authors:  P J Bruns; Y M Sanford
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

4.  Adolescence in Tetrahymena thermophila.

Authors:  M B Rogers; K M Karrer
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

5.  Cytogamy: An Inducible, Alternate Pathway of Conjugation in TETRAHYMENA THERMOPHILA.

Authors:  E Orias; E P Hamilton
Journal:  Genetics       Date:  1979-04       Impact factor: 4.562

Review 6.  Induction and isolation of mutants in Tetrahymena.

Authors:  E Orias; P J Bruns
Journal:  Methods Cell Biol       Date:  1976       Impact factor: 1.441

7.  Osmotic shock prevents nuclear exchange and produces whole-genome homozygotes in conjugating Tetrahymena.

Authors:  E Orias; E P Hamilton; M Flacks
Journal:  Science       Date:  1979-02-16       Impact factor: 47.728

8.  Genomic exclusion: a rapid means for inducing homozygous diploid lines in Tetrahymena pyriformis, syngen 1.

Authors:  S L Allen
Journal:  Science       Date:  1967-02-03       Impact factor: 47.728

9.  Phenylketonuric Tetrahymena: phenylalanine hydroxylase mutants and other tyrosine auxotrophs.

Authors:  Y M Sanford; E Orias
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

10.  Cytofluorimetric analysis of nuclear DNA during meiosis, fertilization and macronuclear development in the ciliate Tetrahymena pyriformis, syngen 1.

Authors:  F P Doerder; L E Debault
Journal:  J Cell Sci       Date:  1975-05       Impact factor: 5.285
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  14 in total

Review 1.  Tetrahymena as a Unicellular Model Eukaryote: Genetic and Genomic Tools.

Authors:  Marisa D Ruehle; Eduardo Orias; Chad G Pearson
Journal:  Genetics       Date:  2016-06       Impact factor: 4.562

2.  Tetrahymena: the key to the genetic analysis of the regulated pathway of polypeptide secretion?

Authors:  J C Hutton
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-30       Impact factor: 11.205

3.  Tetrahymena in the laboratory: strain resources, methods for culture, maintenance, and storage.

Authors:  Donna M Cassidy-Hanley
Journal:  Methods Cell Biol       Date:  2012       Impact factor: 1.441

4.  Genome-wide characterization of Tetrahymena thermophila chromosome breakage sites. II. Physical and genetic mapping.

Authors:  Donna Cassidy-Hanley; Yelena Bisharyan; Vladimir Fridman; Joseph Gerber; Cindy Lin; Eduardo Orias; Judith D Orias; Hilary Ryder; Linh Vong; Eileen P Hamilton
Journal:  Genetics       Date:  2005-06-14       Impact factor: 4.562

5.  Genome-wide characterization of tetrahymena thermophila chromosome breakage sites. I. Cloning and identification of functional sites.

Authors:  Eileen Hamilton; Peter Bruns; Cindy Lin; Virginia Merriam; Eduardo Orias; Linh Vong; Donna Cassidy-Hanley
Journal:  Genetics       Date:  2005-06-14       Impact factor: 4.562

6.  A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction.

Authors:  D Cassidy-Hanley; M C Yao; P J Bruns
Journal:  Genetics       Date:  1994-05       Impact factor: 4.562

7.  Analysis of exocytosis mutants indicates close coupling between regulated secretion and transcription activation in Tetrahymena.

Authors:  A Haddad; A P Turkewitz
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-30       Impact factor: 11.205

8.  Core formation and the acquisition of fusion competence are linked during secretory granule maturation in Tetrahymena.

Authors:  Grant R Bowman; Nels C Elde; Garry Morgan; Mark Winey; Aaron P Turkewitz
Journal:  Traffic       Date:  2005-04       Impact factor: 6.215

9.  An rRNA variable region has an evolutionarily conserved essential role despite sequence divergence.

Authors:  R Sweeney; L Chen; M C Yao
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272

10.  Communication between parental and developing genomes during tetrahymena nuclear differentiation is likely mediated by homologous RNAs.

Authors:  Douglas L Chalker; Patrick Fuller; Meng-Chao Yao
Journal:  Genetics       Date:  2004-09-30       Impact factor: 4.562
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