Literature DB >> 8056326

A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction.

D Cassidy-Hanley1, M C Yao, P J Bruns.   

Abstract

A method for mapping DNA sequences to specific germinal chromosomes in the ciliated protozoan Tetrahymena thermophila has been developed. This mapping technique (PCR mapping) utilizes the polymerase chain reaction and template DNA derived from nullisomic strains to directly assign micronuclear DNA sequences to specific micronuclear chromosomes. Using this technique, a number of unique sequences and short repetitive sequences flanked by unique sequences have been mapped to four of the five germinal chromosomes.

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Year:  1994        PMID： 8056326      PMCID： PMC1205958     

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  25 in total

1.  Chromosomal localization of an exocytosis mutant in Tetrahymena thermophila.

Authors:  L K Bleyman; B H Satir
Journal:  J Protozool       Date:  1990 Nov-Dec

2.  Nullisomic tetrahymena: eliminating germinal chromosomes.

Authors:  P J Bruns; T E Brussard
Journal:  Science       Date:  1981-07-31       Impact factor: 47.728

3.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors:  J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

4.  Tandemly repeated hexanucleotide at Tetrahymena rDNA free end is generated from a single copy during development.

Authors:  B O King; M C Yao
Journal:  Cell       Date:  1982-11       Impact factor: 41.582

5.  Ribosomal RNA gene amplification in Tetrahymena may be associated with chromosome breakage and DNA elimination.

Authors:  M C Yao
Journal:  Cell       Date:  1981-06       Impact factor: 41.582

Review 6.  Genome organization and reorganization in Tetrahymena.

Authors:  M A Gorovsky
Journal:  Annu Rev Genet       Date:  1980       Impact factor: 16.830

7.  Nucleotide sequence structure and consistency of a developmentally regulated DNA deletion in Tetrahymena thermophila.

Authors:  C F Austerberry; M C Yao
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

8.  Chromosome-designated mutation selection in Tetrahymena thermophila.

Authors:  M I Altschuler; P J Bruns
Journal:  Genetics       Date:  1984-03       Impact factor: 4.562

9.  RNA and protein synthesis during meiotic prophase in Tetrahymena thermophila.

Authors:  D W Martindale; C D Allis; P J Bruns
Journal:  J Protozool       Date:  1985-11

10.  DNA elimination in Tetrahymena: a developmental process involving extensive breakage and rejoining of DNA at defined sites.

Authors:  M C Yao; J Choi; S Yokoyama; C F Austerberry; C H Yao
Journal:  Cell       Date:  1984-02       Impact factor: 41.582
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  11 in total

1.  Nongenic, bidirectional transcription precedes and may promote developmental DNA deletion in Tetrahymena thermophila.

Authors:  D L Chalker; M C Yao
Journal:  Genes Dev       Date:  2001-05-15       Impact factor: 11.361

2.  The highly conserved family of Tetrahymena thermophila chromosome breakage elements contains an invariant 10-base-pair core.

Authors:  Eileen P Hamilton; Sondra Williamson; Sandra Dunn; Virginia Merriam; Cindy Lin; Linh Vong; Jessica Russell-Colantonio; Eduardo Orias
Journal:  Eukaryot Cell       Date:  2006-04

3.  Genome-wide characterization of tetrahymena thermophila chromosome breakage sites. I. Cloning and identification of functional sites.

Authors:  Eileen Hamilton; Peter Bruns; Cindy Lin; Virginia Merriam; Eduardo Orias; Linh Vong; Donna Cassidy-Hanley
Journal:  Genetics       Date:  2005-06-14       Impact factor: 4.562

4.  A small family of elements with long inverted repeats is located near sites of developmentally regulated DNA rearrangement in Tetrahymena thermophila.

Authors:  J M Wells; J L Ellingson; D M Catt; P J Berger; K M Karrer
Journal:  Mol Cell Biol       Date:  1994-09       Impact factor: 4.272

5.  Flanking regulatory sequences of the Tetrahymena R deletion element determine the boundaries of DNA rearrangement.

Authors:  D L Chalker; A La Terza; A Wilson; C D Kroenke; M C Yao
Journal:  Mol Cell Biol       Date:  1999-08       Impact factor: 4.272

6.  Identification, mapping and linkage analysis of randomly amplified DNA polymorphisms in Tetrahymena thermophila.

Authors:  J H Brickner; T J Lynch; D Zeilinger; E Orias
Journal:  Genetics       Date:  1996-06       Impact factor: 4.562

7.  Mapping three classical isozyme loci in tetrahymena: meiotic linkage of EstA to the ChxA linkage group.

Authors:  S L Allen; D Zeilinger; E Orias
Journal:  Genetics       Date:  1996-12       Impact factor: 4.562

8.  The conjugation-specific Die5 protein is required for development of the somatic nucleus in both Paramecium and Tetrahymena.

Authors:  Atsushi Matsuda; Annie Wan-Yi Shieh; Douglas L Chalker; James D Forney
Journal:  Eukaryot Cell       Date:  2010-05-21

9.  Role of micronucleus-limited DNA in programmed deletion of mse2.9 during macronuclear development of Tetrahymena thermophila.

Authors:  Jeffrey S Fillingham; Ronald E Pearlman
Journal:  Eukaryot Cell       Date:  2004-04

10.  Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome.

Authors:  Eileen P Hamilton; Aurélie Kapusta; Piroska E Huvos; Shelby L Bidwell; Nikhat Zafar; Haibao Tang; Michalis Hadjithomas; Vivek Krishnakumar; Jonathan H Badger; Elisabet V Caler; Carsten Russ; Qiandong Zeng; Lin Fan; Joshua Z Levin; Terrance Shea; Sarah K Young; Ryan Hegarty; Riza Daza; Sharvari Gujja; Jennifer R Wortman; Bruce W Birren; Chad Nusbaum; Jainy Thomas; Clayton M Carey; Ellen J Pritham; Cédric Feschotte; Tomoko Noto; Kazufumi Mochizuki; Romeo Papazyan; Sean D Taverna; Paul H Dear; Donna M Cassidy-Hanley; Jie Xiong; Wei Miao; Eduardo Orias; Robert S Coyne
Journal:  Elife       Date:  2016-11-28       Impact factor: 8.140
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