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Literature DB >> 277935

Mass isolation and fertility testing of temperature-sensitive mutants in Tetrahymena.

Abstract

A set of 239 heat-sensitive (38 degrees) and 16 cold-sensitive (18 degrees) conditional mutants of Tetrahymena has been generated by combining techniques to manipulate large numbers of clones with a method for the selection of self-fertilized cells after mutagen treatment. A simple technique is presented for determining the fertility of individual clones; 179 of the clones in this set (71%) are fertile. The fertile conditional mutants have already been shown to have lesions in a number of diverse functions, including nucleic acid metabolism, mobility, cell cycle, and cortical pattern.

Mesh：

Year: 1978 PMID： 277935 PMCID： PMC392774 DOI： 10.1073/pnas.75.7.3355

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

14 in total

1. Analysis of RNA and protein synthesis in temperature-sensitive mutants of Tetrahymena pyriformis.

Authors: R L Hallberg; L R Gritz; P Sylvan
Journal: Mol Gen Genet Date: 1978-01-17

2. Characterization of the species of the Tetrahymena pyriformis complex.

Authors: D L Nanney; J W McCoy
Journal: Trans Am Microsc Soc Date: 1976-10

3. Proceedings: The preservation of ciliated protozoa at low temperature.

Authors: E M Simon; M V Schneller
Journal: Cryobiology Date: 1973-11 Impact factor: 2.487

4. Pair formation in tetrahymena pyriformis, an inducible developmental system.

Authors: P J Bruns; T B Brussard
Journal: J Exp Zool Date: 1974-06

5. Positive selection for mating with functional heterokaryons in Tetrahymena pyriformis.

Authors: P J Bruns; T B Brussard
Journal: Genetics Date: 1974-11 Impact factor: 4.562

Review 6. Aging and long-term temporal regulation in ciliated protozoa. A critical review.

Authors: D L Nanney
Journal: Mech Ageing Dev Date: 1974 Jul-Aug Impact factor: 5.432

7. Freezing and storage in liquid nitrogen of axenically and monoxenically cultivated Tetrahymena pyriformis.

Authors: E M Simon
Journal: Cryobiology Date: 1972-04 Impact factor: 2.487

8. Isolation of homozygous mutants after induced self-fertilization in Tetrahymena.

Authors: P J Bruns; T B Brussard; A B Kavka
Journal: Proc Natl Acad Sci U S A Date: 1976-09 Impact factor: 11.205

9. Genetics of cycloheximide resistance in Tetrahymena.

Authors: L K Bleyman; P J Bruns
Journal: Genetics Date: 1977-10 Impact factor: 4.562

10. Causal relations among cell cycle processes in Tetrahymena pyriformis. An analysis employing temperature-sensitive mutants.

Authors: J Frankel; L M Jenkins; L E DeBault
Journal: J Cell Biol Date: 1976-10 Impact factor: 10.539

11 in total

5. Mutants of TETRAHYMENA THERMOPHILA with Temperature-Sensitive Food Vacuole Formation. I. Isolation and Genetic Characterization.

Authors: P B Suhr-Jessen; E Orias
Journal: Genetics Date: 1979-08 Impact factor: 4.562

10. Uniparental cytogamy: a novel method for bringing micronuclear mutations of Tetrahymena into homozygous macronuclear expression with precocious sexual maturity.

Authors: E S Cole; P J Bruns
Journal: Genetics Date: 1992-12 Impact factor: 4.562

Mass isolation and fertility testing of temperature-sensitive mutants in Tetrahymena.

1. Analysis of RNA and protein synthesis in temperature-sensitive mutants of Tetrahymena pyriformis.

2. Characterization of the species of the Tetrahymena pyriformis complex.

3. Proceedings: The preservation of ciliated protozoa at low temperature.

4. Pair formation in tetrahymena pyriformis, an inducible developmental system.

5. Positive selection for mating with functional heterokaryons in Tetrahymena pyriformis.

Review 6. Aging and long-term temporal regulation in ciliated protozoa. A critical review.

7. Freezing and storage in liquid nitrogen of axenically and monoxenically cultivated Tetrahymena pyriformis.

8. Isolation of homozygous mutants after induced self-fertilization in Tetrahymena.

9. Genetics of cycloheximide resistance in Tetrahymena.

10. Causal relations among cell cycle processes in Tetrahymena pyriformis. An analysis employing temperature-sensitive mutants.

1. Pronuclear fusion failure: an alternate conjugational pathway in Tetrahymena thermophila, induced by vinblastine.

Review 2. Tetrahymena as a Unicellular Model Eukaryote: Genetic and Genomic Tools.

3. Selective mirror-image reversal of ciliary patterns inTetrahymena thermophila homozygous for ajanus mutation.

4. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.

5. Mutants of TETRAHYMENA THERMOPHILA with Temperature-Sensitive Food Vacuole Formation. I. Isolation and Genetic Characterization.

Review 6. Formation and positioning of surface-related structures in protozoa.

7. Phenylketonuric Tetrahymena: phenylalanine hydroxylase mutants and other tyrosine auxotrophs.

8. Chromosome-designated mutation selection in Tetrahymena thermophila.

9. Isolation and genetic characterization of a mutation affecting ribosomal resistance to cycloheximide in Tetrahymena.

10. Uniparental cytogamy: a novel method for bringing micronuclear mutations of Tetrahymena into homozygous macronuclear expression with precocious sexual maturity.