| Literature DB >> 14273659 |
C K HUSTON, P W ALBRO, G B GRINDEY.
Abstract
Huston, Charles K. (Fort Detrick, Frederick, Md.), Phillip W. Albro, and Gerald B. Grindey. Lipids of Sarcina lutea. III. Composition of the complex lipids. J. Bacteriol. 89:768-775. 1965.-The complex lipids from a strain of Sarcina lutea were isolated and separated into fractions on diethylaminoethyl cellulose acetate and silicic acid columns. These fractions were monitored in several thin-layer chromatography systems. The various lipid types were characterized by their behavior in thin-layer systems and by an analysis of their hydrolysis products. The fatty acid composition of the column fractions was determined by gas-liquid chromatography. A number of components (13) were separated by thin-layer chromatography and characterized. The major components were polyglycerol phosphatide (17.0%), lipoamino acids (15.1%), phosphatidyl glycerol (13.8%), and an incompletely characterized substance (15.0%). Minor constituents included phosphatidyl inositol (5.5%), phosphatidic acid (4.2%), phosphatidyl serine (2.0%), and phosphatidyl choline (1.0%). No phosphatidyl ethanolamine was observed.Entities:
Keywords: AMINO ACIDS; CHEMISTRY; CHROMATOGRAPHY; EXPERIMENTAL LAB STUDY; FATTY ACIDS; LECITHINS; LIPIDS; PHOSPHOINOSITIDES; SARCINA
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Year: 1965 PMID: 14273659 PMCID: PMC277535 DOI: 10.1128/jb.89.3.768-775.1965
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490