Literature DB >> 1422153

A new 3D HN(CA)HA experiment for obtaining fingerprint HN-Halpha peaks in 15N- and 13C-labeled proteins.

R T Clubb1, V Thanabal, G Wagner.   

Abstract

A new 3D 1H-15N-13C triple resonance experiment is presented that provides in-phase absorptive cross peaks between amide protons and alpha-protons of the same and the sequentially preceding residue. The experiment yields similar connectivities as those described previously by Montelione and Wagner (1990a) (J. Magn. Reson., 87, 183-188) and Kay et al. (1991) (J. Magn. Reson., 91, 84-92). However, the pulse sequence was designed to minimize the time that transverse coherence of the 13Calpha nucleus is present, since this nucleus has the shortest transverse relaxation time of all the nuclei involved in these experiments. This is achieved by using a coherence transfer pathway from 1HN to 15N, 13Calpha, 1Halpha and back to the 1HN. In the sequence described, transverse 13Calpha coherence is present only for a length of ca. 1/1J(Calpha-Halpha). This reduces loss of signal due to transverse relaxation. We tested the technique on uniformly 15N- and 13C-enriched T4 lysozyme.

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Year:  1992        PMID： 1422153     DOI： 10.1007/bf01875531

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  6 in total

1.  Studies of J-connectives and selective 1H-1H Overhauser effects in H2O solutions of biological macromolecules by two-dimensional NMR experiments.

Authors:  A Kumar; G Wagner; R R Ernst; K Wüthrich
Journal:  Biochem Biophys Res Commun       Date:  1980-10-16       Impact factor: 3.575

2.  Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Basic pancreatic trypsin inhibitor.

Authors:  G Wagner; K Wüthrich
Journal:  J Mol Biol       Date:  1982-03-05       Impact factor: 5.469

3.  Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins.

Authors:  D Marion; K Wüthrich
Journal:  Biochem Biophys Res Commun       Date:  1983-06-29       Impact factor: 3.575

4.  Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta.

Authors:  D Marion; P C Driscoll; L E Kay; P T Wingfield; A Bax; A M Gronenborn; G M Clore
Journal:  Biochemistry       Date:  1989-07-25       Impact factor: 3.162

5.  Assignment of the backbone 1H and 15N NMR resonances of bacteriophage T4 lysozyme.

Authors:  L P McIntosh; A J Wand; D F Lowry; A G Redfield; F W Dahlquist
Journal:  Biochemistry       Date:  1990-07-10       Impact factor: 3.162

6.  A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

Authors:  M Ikura; L E Kay; A Bax
Journal:  Biochemistry       Date:  1990-05-15       Impact factor: 3.162
  6 in total
  34 in total

1.  Structural and functional characterization of CFE88: evidence that a conserved and essential bacterial protein is a methyltransferase.

Authors:  Keith L Constantine; Stanley R Krystek; Matthew D Healy; Michael L Doyle; Nathan O Siemers; Jane Thanassi; Ning Yan; Dianlin Xie; Valentina Goldfarb; Joseph Yanchunas; Li Tao; Brian A Dougherty; Bennett T Farmer
Journal:  Protein Sci       Date:  2005-06       Impact factor: 6.725

2.  Unambiguous assignment of NMR protein backbone signals with a time-shared triple-resonance experiment.

Authors:  Dominique P Frueh; Haribabu Arthanari; Gerhard Wagner
Journal:  J Biomol NMR       Date:  2005-11       Impact factor: 2.835

3.  Complete 1H, 13C, and 15N NMR resonance assignments and secondary structure of human glutaredoxin in the fully reduced form.

Authors:  C Sun; A Holmgren; J H Bushweller
Journal:  Protein Sci       Date:  1997-02       Impact factor: 6.725

4.  Conformation of an Shc-derived phosphotyrosine-containing peptide complexed with the Grb2 SH2 domain.

Authors:  K Ogura; S Tsuchiya; H Terasawa; S Yuzawa; H Hatanaka; V Mandiyan; J Schlessinger; F Inagaki
Journal:  J Biomol NMR       Date:  1997-10       Impact factor: 2.835

5.  (1)H, (13)C and (15)N NMR backbone assignments of the 269-residue serine protease PB92 from Bacillus alcalophilus.

Authors:  R H Fogh; D Schipper; R Boelens; R Kaptein
Journal:  J Biomol NMR       Date:  1994-01       Impact factor: 2.835

6.  Minimisation of sensitivity losses due to the use of gradient pulses in triple-resonance NMR of proteins.

Authors:  J Stonehouse; R T Clowes; G L Shaw; J Keeler; E D Laue
Journal:  J Biomol NMR       Date:  1995-04       Impact factor: 2.835

7.  Application of multiple-quantum line narrowing with simultaneous 1H and 13C constant-time scalar-coupling evolution in PFG-HACANH and PFG-HACA(CO)NH triple-resonance experiments.

Authors:  G V Swapna; C B Rios; Z Shang; G T Montelione
Journal:  J Biomol NMR       Date:  1997-01       Impact factor: 2.835

8.  A variable light domain fluorogen activating protein homodimerizes to activate dimethylindole red.

Authors:  Nina Senutovitch; Robyn L Stanfield; Shantanu Bhattacharyya; Gordon S Rule; Ian A Wilson; Bruce A Armitage; Alan S Waggoner; Peter B Berget
Journal:  Biochemistry       Date:  2012-03-14       Impact factor: 3.162

9.  TCR scanning of peptide/MHC through complementary matching of receptor and ligand molecular flexibility.

Authors:  William F Hawse; Soumya De; Alex I Greenwood; Linda K Nicholson; Jaroslav Zajicek; Evgenii L Kovrigin; David M Kranz; K Christopher Garcia; Brian M Baker
Journal:  J Immunol       Date:  2014-02-12       Impact factor: 5.422

10.  Sequential assignment of the backbone nuclei (1H, 15N and 13C) of c-H-ras p21 (1-166).GDP using a novel 4D NMR strategy.

Authors:  S L Campbell-Burk; P J Domaille; M A Starovasnik; W Boucher; E D Laue
Journal:  J Biomol NMR       Date:  1992-11       Impact factor: 2.835
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