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Literature DB >> 14197891

PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN.

J GERWING, C E DOLMAN, M E REICHMANN, H S BAINS.

Abstract

Gerwing, Julia (The University of British Columbia, Vancouver, British Columbia, Canada), Claude E. Dolman, M. E. Reichmann, and Hardial S. Bains. Purification and molecular weight determination of Clostridium botulinum type E toxin. J. Bacteriol. 88:216-219. 1964.-A method was developed whereby type E botulinus toxin can be obtained in a highly purified state by elution through acidified diethylaminoethyl-cellulose columns. The material thus isolated appears to be electrophoretically and ultracentrifugally homogeneous. A molecular weight of 18,600 was calculated for the toxin.

Entities: Chemical Disease Species

Keywords: CHEMISTRY, ANALYTICAL; CHROMATOGRAPHY; CLOSTRIDIUM BOTULINUM; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; MOLECULAR WEIGHT; TOXINS AND ANTITOXINS; ULTRACENTRIFUGATION

Mesh：

Substances：

Year: 1964 PMID： 14197891 PMCID： PMC277279 DOI： 10.1128/jb.88.1.216-219.1964

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

11 in total

1. Studies on toxin production of Clostridium botulinum type E. III. Characterization of toxin precursor.

Authors: G SAKAGUCHI; S SAKAGUCHI
Journal: J Bacteriol Date: 1959-07 Impact factor: 3.490

2. Activation phenomenon of Clostridium botulinum type E toxin.

Authors: J GERWING; C E DOLMAN; D A ARNOTT
Journal: J Bacteriol Date: 1962-08 Impact factor: 3.490

3. Purification and activation of Clostridium botulinum type E toxin.

Authors: J GERWING; C E DOLMAN; D A ARNOTT
Journal: J Bacteriol Date: 1961-05 Impact factor: 3.490

4. Studies on immunity to toxins of Clostridium botulinum. III. Preparation, purification, and detoxification of type E toxin.

Authors: M GORDON; M A FIOCK; A YARINSKY; J T DUFF
Journal: J Bacteriol Date: 1957-10 Impact factor: 3.490

5. Activation of Clostridium botulinum type E toxin by trypsin.

Authors: J T DUFF; G G WRIGHT; A YARINSKY
Journal: J Bacteriol Date: 1956-10 Impact factor: 3.490

6. Isolation and sedimentation study of low molecular weight forms of type A Botulins toxin.

Authors: J WAGMAN
Journal: Arch Biochem Biophys Date: 1954-05 Impact factor: 4.013

7. The Isolation of Type B Botulinum Toxin.

Authors: C Lamanna; H N Glassman
Journal: J Bacteriol Date: 1947-11 Impact factor: 3.490

8. The behavior of the bolulinus toxins in the ultracentrifuge.

Authors: J WAGMAN; J B BATEMAN
Journal: Arch Biochem Biophys Date: 1951-05 Impact factor: 4.013

9. The Purification and Crystallization of Clostridium botulinum Type A Toxin.

Authors: C Lamanna; O E McElroy; H W Eklund
Journal: Science Date: 1946-05-17 Impact factor: 47.728

10. Studies on the toxin production of Clostridium botulinum type E. I. A strain of genus Clostridium having the action to promote type E botulinal toxin production in a mixed culture.

Authors: G SAKAGUCHI; Y TOHYAMA
Journal: Jpn J Med Sci Biol Date: 1955-06

8 in total

1. Molecular weight of type a botulinum toxin.

Authors: J N Knox; W P Brown; L Spero
Journal: Infect Immun Date: 1970-02 Impact factor: 3.441

2. Clostridium botulinum type E toxin: effect of pH and method of purification on molecular weight.

Authors: H S Sacks; S V Covert
Journal: Appl Microbiol Date: 1974-09

3. Intracellular synthesis of Clostridium botulinum type B toxin. I. Demonstration of toxin synthesis and sedimentation studies on toxic products.

Authors: J Gerwing; R W Morrell; R M Nitz
Journal: J Bacteriol Date: 1968-01 Impact factor: 3.490