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Literature DB >> 14293025

ISOLATION AND CHARACTERIZATION OF A TOXIC MOIETY OF LOW MOLECULAR WEIGHT FROM CLOSTRIDIUM BOTULINUM TYPE A.

Abstract

Gerwing, Julia (The University of British Columbia, Vancouver, B.C., Canada), Claude E. Dolman, and Hardial S. Bains. Isolation and characterization of a toxic moiety of low molecular weight from Clostridium botulinum type A. J. Bacteriol. 89:1383-1386. 1965.-A toxic moiety of low molecular weight has been isolated from a type A strain of Clostridium botulinum, by a method involving ammonium sulfate precipitation and elution through diethylaminoethyl cellulose at pH 5.6. By means of electrophoresis and ultracentrifugation, the toxic substance was shown to be homogeneous; a molecular weight of 12,200 was calculated.

Entities: Chemical Disease Species

Keywords: AMMONIUM COMPOUNDS; BIOLOGICAL ASSAY; CHROMATOGRAPHY; CLOSTRIDIUM BOTULINUM; DEAE-CELLULOSE; ELECTROPHORESIS; EXPERIMENTAL LAB STUDY; HYDROGEN-ION CONCENTRATION; MICE; MOLECULAR WEIGHT; PRECIPITATION; SULFATES; TOXINS AND ANTITOXINS; ULTRACENTRIFUGATION

Mesh：

Substances：

Year: 1965 PMID： 14293025 PMCID： PMC277655 DOI： 10.1128/jb.89.5.1383-1386.1965

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

5 in total

1. USE OF KCN FOR STABILIZATION OF COLOR IN DIRECT NESSLERIZATION OF KJELDAHL DIGESTS.

Authors: O MINARI; D B ZILVERSMIT
Journal: Anal Biochem Date: 1963-10 Impact factor: 3.365

2. Studies on immunity to toxins of Clostridium botulinum. I. A simplified procedure for isolation of type A toxin.

Authors: J T DUFF; G G WRIGHT; J KLERER; D E MOORE; R H BIBLER
Journal: J Bacteriol Date: 1957-01 Impact factor: 3.490

3. Isolation and sedimentation study of low molecular weight forms of type A Botulins toxin.

Authors: J WAGMAN
Journal: Arch Biochem Biophys Date: 1954-05 Impact factor: 4.013

4. The Purification and Crystallization of Clostridium botulinum Type A Toxin.

Authors: C Lamanna; O E McElroy; H W Eklund
Journal: Science Date: 1946-05-17 Impact factor: 47.728

5. PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN.

Authors: J GERWING; C E DOLMAN; M E REICHMANN; H S BAINS
Journal: J Bacteriol Date: 1964-07 Impact factor: 3.490

5 in total

8 in total

1. Molecular weight of type a botulinum toxin.

Authors: J N Knox; W P Brown; L Spero
Journal: Infect Immun Date: 1970-02 Impact factor: 3.441

2. Clostridium botulinum type E toxin: effect of pH and method of purification on molecular weight.

Authors: H S Sacks; S V Covert
Journal: Appl Microbiol Date: 1974-09

3. Intracellular synthesis of Clostridium botulinum type B toxin. I. Demonstration of toxin synthesis and sedimentation studies on toxic products.

Authors: J Gerwing; R W Morrell; R M Nitz
Journal: J Bacteriol Date: 1968-01 Impact factor: 3.490

ISOLATION AND CHARACTERIZATION OF A TOXIC MOIETY OF LOW MOLECULAR WEIGHT FROM CLOSTRIDIUM BOTULINUM TYPE A.

1. USE OF KCN FOR STABILIZATION OF COLOR IN DIRECT NESSLERIZATION OF KJELDAHL DIGESTS.

2. Studies on immunity to toxins of Clostridium botulinum. I. A simplified procedure for isolation of type A toxin.

3. Isolation and sedimentation study of low molecular weight forms of type A Botulins toxin.

4. The Purification and Crystallization of Clostridium botulinum Type A Toxin.

5. PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN.

1. Molecular weight of type a botulinum toxin.

2. Clostridium botulinum type E toxin: effect of pH and method of purification on molecular weight.

3. Intracellular synthesis of Clostridium botulinum type B toxin. I. Demonstration of toxin synthesis and sedimentation studies on toxic products.

4. Significance of 12S toxin of Clostridium botulinum type E.

5. Amino acid composition of crystalline botulinum toxin, type A.

6. Amino acid composition of Clostridium botulinum type A toxin.

7. A food-poisoning incident caused by Clostridium botulinum toxin A in Japan.

8. Homogeneity and molecular weight of toxin of Clostridium botulinum type B.