Literature DB >> 1409694

Metal-binding chimeric antibodies expressed in Escherichia coli.

J R Sawyer1, P W Tucker, F R Blattner.   

Abstract

Metallothionein, a well-characterized biological chelator of metals, has been genetically fused to the binding domain of an antibody and expressed in the periplasm of Escherichia coli. Specific delivery of 109Cd to immobilized hapten or to haptenated cells was demonstrated directly in periplasmic extracts. This approach is potentially useful for targeted radiotherapy and diagnostic imaging. We find six to seven atoms of metal per active antigen-combining site. Absence of the Fc portion of the immunoglobulin along with low immunogenicity of metallothionein-metal complexes should reduce immunologic reactions.

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Year:  1992        PMID： 1409694      PMCID： PMC50211          DOI： 10.1073/pnas.89.20.9754

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins.

Authors:  V K Chaudhary; J K Batra; M G Gallo; M C Willingham; D J FitzGerald; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

2.  Preparation of Fv fragment from the mouse myeloma XRPC-25 immunoglobulin possessing anti-dinitrophenyl activity.

Authors:  J Sharon; D Givol
Journal:  Biochemistry       Date:  1976-04-06       Impact factor: 3.162

3.  Replacing the complementarity-determining regions in a human antibody with those from a mouse.

Authors:  P T Jones; P H Dear; J Foote; M S Neuberger; G Winter
Journal:  Nature       Date:  1986 May 29-Jun 4       Impact factor: 49.962

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Escherichia coli secretion of an active chimeric antibody fragment.

Authors:  M Better; C P Chang; R R Robinson; A H Horwitz
Journal:  Science       Date:  1988-05-20       Impact factor: 47.728

6.  Construction and expression of a recombinant antibody-targeted plasminogen activator.

Authors:  J M Schnee; M S Runge; G R Matsueda; N W Hudson; J G Seidman; E Haber; T Quertermous
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

7.  Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli.

Authors:  M A Boss; J H Kenten; C R Wood; J S Emtage
Journal:  Nucleic Acids Res       Date:  1984-05-11       Impact factor: 16.971

8.  Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

Authors:  E Amann; J Brosius; M Ptashne
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

9.  Molecular cloning of the rat metallothionein 1 (MT-1) mRNA sequence.

Authors:  R D Andersen; B W Birren; T Ganz; J E Piletz; H R Herschman
Journal:  DNA       Date:  1983

10.  Metallothionein: a bifunctional chelator for the radiolabeling of biologically active molecules.

Authors:  M M Morelock; G L Tolman
Journal:  Experientia Suppl       Date:  1987
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  6 in total

1.  Recombinant antibody-metallothionein: design and evaluation for radioimmunoimaging.

Authors:  C Das; P V Kulkarni; A Constantinescu; P Antich; F R Blattner; P W Tucker
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-15       Impact factor: 11.205

2.  Metallothionein as a clonable high-density marker for cryo-electron microscopy.

Authors:  Cédric Bouchet-Marquis; Maria Pagratis; Robert Kirmse; Andreas Hoenger
Journal:  J Struct Biol       Date:  2011-10-31       Impact factor: 2.867

Review 3.  Engineering recombinant antibodies for immunotherapy.

Authors:  D Neri; H Petrul; G Roncucci
Journal:  Cell Biophys       Date:  1995-08

4.  Radiometal labeling of recombinant proteins by a genetically engineered minimal chelation site: technetium-99m coordination by single-chain Fv antibody fusion proteins through a C-terminal cysteinyl peptide.

Authors:  A J George; F Jamar; M S Tai; B T Heelan; G P Adams; J E McCartney; L L Houston; L M Weiner; H Oppermann; A M Peters
Journal:  Proc Natl Acad Sci U S A       Date:  1995-08-29       Impact factor: 11.205

5.  A universal antibody-derived targeting agent.

Authors:  R A Spooner; M P Deonarain; P Savage; A A Epenetos
Journal:  Cell Biophys       Date:  1993 Jan-Jun

6.  Genetic incorporation of human metallothionein into the adenovirus protein IX for non-invasive SPECT imaging.

Authors:  J Michael Mathis; Shilpa Bhatia; Alok Khandelwal; Imre Kovesdi; Stephen J Lokitz; Yoshi Odaka; Amol M Takalkar; Tracee Terry; David T Curiel
Journal:  PLoS One       Date:  2011-02-09       Impact factor: 3.240
  6 in total

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