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Literature DB >> 7493398

Engineering recombinant antibodies for immunotherapy.

Abstract

Recombinant antibody fragments binding with high affinity to their target can be obtained either from hybridomas or directly from antibody libraries on filamentous phage. These fragments are devoid of any activity other than antigen binding, and have to be processed and functionalized in order to be suitable for clinical applications. This article presents the authors' view on the procedures and the features that are important for effective transformation of recombinant antibodies into useful immunotherapeutic agents. The topics presented include phage display methodologies, engineering of high-affinity binding, purification, and functionalization strategies of recombinant antibodies.

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Year: 1995 PMID： 7493398 DOI： 10.1007/bf02822526

Source DB: PubMed Journal: Cell Biophys ISSN： 0163-4992

55 in total

1. Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor.

Authors: W Wels; I M Harwerth; M Zwickl; N Hardman; B Groner; N E Hynes
Journal: Biotechnology (N Y) Date: 1992-10

2. In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library.

Authors: H Gram; L A Marconi; C F Barbas; T A Collet; R A Lerner; A S Kang
Journal: Proc Natl Acad Sci U S A Date: 1992-04-15 Impact factor: 11.205

Review 3. Man-made antibodies.

Authors: G Winter; C Milstein
Journal: Nature Date: 1991-01-24 Impact factor: 49.962

4. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

Authors: M Fried; D M Crothers
Journal: Nucleic Acids Res Date: 1981-12-11 Impact factor: 16.971

5. Cure of xenografted human carcinomas by BR96-doxorubicin immunoconjugates.

Authors: P A Trail; D Willner; S J Lasch; A J Henderson; S Hofstead; A M Casazza; R A Firestone; I Hellström; K E Hellström
Journal: Science Date: 1993-07-09 Impact factor: 47.728

6. Bifunctional hybrids between the variable domains of an immunoglobulin and the maltose-binding protein of Escherichia coli: production, purification and antigen binding.

Authors: F Brégégère; J Schwartz; H Bedouelle
Journal: Protein Eng Date: 1994-02

7. Metal-binding chimeric antibodies expressed in Escherichia coli.

Authors: J R Sawyer; P W Tucker; F R Blattner
Journal: Proc Natl Acad Sci U S A Date: 1992-10-15 Impact factor: 11.205

8. Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli.

Authors: P J Schatz
Journal: Biotechnology (N Y) Date: 1993-10

9. Cancer Research Campaign operation manual for control recommendations for products derived from recombinant DNA technology prepared for investigational administration to patients with cancer in phase I trials.

Authors: R H Begent; K A Chester; T Connors; D Crowther; B Fox; E Griffiths; T A Hince; J A Ledermann; J G McVie; P Minor
Journal: Eur J Cancer Date: 1993 Impact factor: 9.162

Engineering recombinant antibodies for immunotherapy.

1. Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor.

2. In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library.

Review 3. Man-made antibodies.

4. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

5. Cure of xenografted human carcinomas by BR96-doxorubicin immunoconjugates.

6. Bifunctional hybrids between the variable domains of an immunoglobulin and the maltose-binding protein of Escherichia coli: production, purification and antigen binding.

7. Metal-binding chimeric antibodies expressed in Escherichia coli.

8. Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli.

9. Cancer Research Campaign operation manual for control recommendations for products derived from recombinant DNA technology prepared for investigational administration to patients with cancer in phase I trials.

10. Calmodulin and calmodulin-regulated protein kinases as transducers of intracellular calcium signals.

1. Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures.

2. Efficient one-step direct labelling of recombinant antibodies with technetium-99m.

Review 3. Capillary electrophoresis-based immunoassays: principles and quantitative applications.

4. Natural and man-made V-gene repertoires for antibody discovery.

Review 5. Single Domain Antibodies as New Biomarker Detectors.

6. PuLSE: Quality control and quantification of peptide sequences explored by phage display libraries.