Literature DB >> 3285471

Escherichia coli secretion of an active chimeric antibody fragment.

M Better1, C P Chang, R R Robinson, A H Horwitz.   

Abstract

A chimeric mouse-human Fab protein that binds specifically to the human carcinoma cell line C3347 has been expressed and secreted from Escherichia coli. This molecule, which contains functionally assembled kappa and Fd proteins, binds as effectively to sites on the surface of C3347 cells as Fab fragments prepared proteolytically from whole chimeric or mouse antibody. The production in Escherichia coli of foreign heterodimeric protein reagents, such as Fab, should prove useful in the management of human disease.

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Year:  1988        PMID： 3285471     DOI： 10.1126/science.3285471

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  77 in total

Review 1.  Generation of recombinant antibodies.

Authors:  S M Kipriyanov; M Little
Journal:  Mol Biotechnol       Date:  1999-09       Impact factor: 2.695

2.  Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries.

Authors:  D Patel; S Vitovski; H J Senior; M D Edge; R C Hockney; M J Dempsey; J R Sayers
Journal:  Biochem J       Date:  2001-08-01       Impact factor: 3.857

3.  Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antibodies designed with metal-binding domains.

Authors:  Marek Malecki; Annie Hsu; Lynn Truong; Sylvia Sanchez
Journal:  Proc Natl Acad Sci U S A       Date:  2001-12-26       Impact factor: 11.205

4.  Single-domain antibody fragments with high conformational stability.

Authors:  Mireille Dumoulin; Katja Conrath; Annemie Van Meirhaeghe; Filip Meersman; Karel Heremans; Leon G J Frenken; Serge Muyldermans; Lode Wyns; Andre Matagne
Journal:  Protein Sci       Date:  2002-03       Impact factor: 6.725

5.  Preparation of recombinant human monoclonal antibody Fab fragments specific for Entamoeba histolytica.

Authors:  H Tachibana; X J Cheng; K Watanabe; M Takekoshi; F Maeda; S Aotsuka; Y Kaneda; T Takeuchi; S Ihara
Journal:  Clin Diagn Lab Immunol       Date:  1999-05

Review 6.  Generation and production of engineered antibodies.

Authors:  Sergey M Kipriyanov; Fabrice Le Gall
Journal:  Mol Biotechnol       Date:  2004-01       Impact factor: 2.695

Review 7.  Recombinant human monoclonal antibodies. Basic principles of the immune system transferred to E. coli.

Authors:  P Fuchs; S Dübel; F Breitling; M Braunagel; I Klewinghaus; M Little
Journal:  Cell Biophys       Date:  1992 Aug-Dec

Review 8.  Recombinant antibodies for the diagnosis and treatment of cancer.

Authors:  Jürgen Krauss
Journal:  Mol Biotechnol       Date:  2003-09       Impact factor: 2.695

9.  In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells.

Authors:  M J Embleton; G Gorochov; P T Jones; G Winter
Journal:  Nucleic Acids Res       Date:  1992-08-11       Impact factor: 16.971

10.  Translational fusion and redirection to thylakoid lumen as strategies to improve the accumulation of a camelid antibody fragment in transplastomic tobacco.

Authors:  Ezequiel M Lentz; Lorena Garaicoechea; E Federico Alfano; Viviana Parreño; Andrés Wigdorovitz; Fernando F Bravo-Almonacid
Journal:  Planta       Date:  2012-04-24       Impact factor: 4.116
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