Literature DB >> 1369189

Characterization of Zymomonas mobilis alkaline phosphatase activity in Escherichia coli.

T Karunakaran1, P Gunasekaran.   

Abstract

Zymomonas mobilis phoA gene encoding alkaline phosphatase was expressed in Escherichia coli CC118 carrying the recombinant plasmid pZAP1. The pH optimum for this enzyme was 9.0 and showed a peak activity at 42 degrees C. This enzyme required Zn2+ for its catalytic activity; however, Mg2+ or Ca2+ significantly affected the activity. This enzyme was found to be ethanolabile, and ethanol inhibition was reversed by addition of Zn2+. Kinetics of Z. mobilis alkaline phosphatase production in E. coli CC118 (pZAP1) showed that the enzyme activity was growth associated and localized in the cellular fraction, and the maximum activity was found in the stationary phase.

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Year:  1992        PMID： 1369189     DOI： 10.1007/bf01570081

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  6 in total

1.  Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages.

Authors:  E Brickman; J Beckwith
Journal:  J Mol Biol       Date:  1975-08-05       Impact factor: 5.469

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Authors:  J Swings; J De Ley
Journal:  Bacteriol Rev       Date:  1977-03

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Authors:  J L Pond; C K Eddy; K F Mackenzie; T Conway; D J Borecky; L O Ingram
Journal:  J Bacteriol       Date:  1989-02       Impact factor: 3.490

4.  Extracellular Ca2(+)-dependent inducible alkaline phosphatase from extremely halophilic archaebacterium Haloarcula marismortui.

Authors:  S Goldman; K Hecht; H Eisenberg; M Mevarech
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

5.  Content and composition of hopanoids in Zymomonas mobilis under various growth conditions.

Authors:  M A Hermans; B Neuss; H Sahm
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

6.  TnphoA: a transposon probe for protein export signals.

Authors:  C Manoil; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205
  6 in total
  1 in total

1.  Characterisation of haemolytic activity from Aeromonas caviae.

Authors:  T Karunakaran; B G Devi
Journal:  Epidemiol Infect       Date:  1994-04       Impact factor: 2.451
  1 in total

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