Literature DB >> 1367799

Expression of streptomycete cholesterol oxidase in Escherichia coli.

D K Solaiman1, G A Somkuti.   

Abstract

A streptomycete gene coding for extracellular cholesterol oxidase (choA) was subcloned and expressed in Escherichia coli. The pUCO series recombinants were obtained by inserting the choA gene into the unique KpnI site of pUC19 vector. Expression was observed with pUCO192A and pUCO193 constructs in which the cloned gene(s) were aligned with the upstream lacZ promoter. Isopropyl beta-D-thioglucopyranoside (IPTG) enhanced this expression up to 2.5-fold. Specific Cho activity in the cell extracts of the stable pUCO193 transformant were 0.004 U and 0.007 U per mg protein without and with IPTG induction, respectively. Cho activity was detected in the spent medium of this culture, suggesting possible secretion of the enzyme.

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Year:  1991        PMID： 1367799     DOI： 10.1007/bf01576063

Source DB:  PubMed          Journal:  J Ind Microbiol        ISSN： 0169-4146


  15 in total

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Review 6.  Export of protein: a biochemical view.

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8.  An operon containing the genes for cholesterol oxidase and a cytochrome P-450-like protein from a Streptomyces sp.

Authors:  M Horii; T Ishizaki; S Y Paik; T Manome; Y Murooka
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9.  Expression of cloned calf prochymosin cDNA under control of the tryptophan promoter.

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10.  Cloning and expression of a Streptomyces cholesterol oxidase gene in Streptomyces lividans with plasmid pIJ702.

Authors:  Y Murooka; T Ishizaki; O Nimi; N Maekawa
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  6 in total

Review 1.  Review: optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter.

Authors:  R S Donovan; C W Robinson; B R Glick
Journal:  J Ind Microbiol       Date:  1996-03

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Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792

3.  Expression of cho and melC operons by a Streptococcus thermophilus synthetic promoter in Escherichia coli.

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Journal:  Appl Microbiol Biotechnol       Date:  1995 May-Jun       Impact factor: 4.813

4.  Expression of Streptomyces melC and choA genes by a cloned Streptococcus thermophilus promoter STP2201.

Authors:  D K Solaiman; G A Somkuti
Journal:  J Ind Microbiol       Date:  1995-07

5.  Expression optimization of recombinant cholesterol oxidase in Escherichia coli and its purification and characterization.

Authors:  Aliakbar Fazaeli; Abolfazl Golestani; Mostafa Lakzaei; Samaneh Sadat Rasi Varaei; Mahdi Aminian
Journal:  AMB Express       Date:  2018-11-12       Impact factor: 3.298

6.  Expression optimization, purification, and functional characterization of cholesterol oxidase from Chromobacterium sp. DS1.

Authors:  Aliakbar Fazaeli; Abolfazl Golestani; Mostafa Lakzaei; Samaneh Sadat Rasi Varaei; Mahdi Aminian
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  6 in total

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