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Literature DB >> 2828888

Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus subtilis.

Abstract

We measured in Bacillus subtilis the recombination frequency between directly repeated 9 bp sequences flanking the transposon Tn5. Four different repeats were analyzed. They recombined with frequencies between 10(-7) and 10(-6) when they were carried on plasmids, and 150-1500 times less frequently when they were carried on the chromosome. We propose that this difference is due to the generation of single stranded DNA during plasmid replication.

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Year: 1987 PMID： 2828888 DOI： 10.1007/BF00337766

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

33 in total

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

2. Repeated DNA sequences recombine 1,000 times more frequently in a plasmid than in the chromosome of Bacillus subtilis.

Authors: L Jannière; B Niaudet; S D Ehrlich
Journal: Basic Life Sci Date: 1985

3. Rapid purification of plasmid DNAs by hydroxyapatite chromatography.

Authors: A Colman; M J Byers; S B Primrose; A Lyons
Journal: Eur J Biochem Date: 1978-11-02

4. Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.

Authors: P J Farabaugh; U Schmeissner; M Hofer; J H Miller
Journal: J Mol Biol Date: 1978-12-25 Impact factor: 5.469

5. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

7. Extremely large chromosomal deletions are intimately involved in genetic instability and genomic rearrangements in Streptomyces glaucescens.

Authors: A Birch; A Häusler; M Vögtli; W Krek; R Hütter
Journal: Mol Gen Genet Date: 1989-06

Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus subtilis.

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

2. Repeated DNA sequences recombine 1,000 times more frequently in a plasmid than in the chromosome of Bacillus subtilis.

3. Rapid purification of plasmid DNAs by hydroxyapatite chromatography.

4. Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.

5. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

6. Stimulation of precise excision and recombination by conjugal proficient F'plasmids.

7. The intergenic region and the origins for filamentous phage DNA replication.

8. Insertion, excision, and inversion of Tn5.

9. Isolation of plasmid deletion Mutants and study of their instability.

10. Stable gene amplification in the chromosome of Bacillus subtilis.

Review 1. The family of highly interrelated single-stranded deoxyribonucleic acid plasmids.

2. Deletion hot spots in chimeric Escherichia coli plasmids.

3. Mechanisms of deletion formation in Escherichia coli plasmids. II. Deletions mediated by short direct repeats.

4. Factors affecting inverted repeat stimulation of recombination and deletion in Saccharomyces cerevisiae.

5. Stability of reiterated sequences in the Bacillus subtilis chromosome.

6. Ribosomal protein L4 of Lactobacillus rhamnosus LRB alters resistance to macrolides and other antibiotics.

7. Extremely large chromosomal deletions are intimately involved in genetic instability and genomic rearrangements in Streptomyces glaucescens.

8. Efficiency of homologous DNA recombination varies along the Bacillus subtilis chromosome.

Review 9. Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes.

10. In vivo analysis of the plasmid pAM beta 1 resolution system.