Literature DB >> 3466572

Cloning and expression of a Streptomyces cholesterol oxidase gene in Streptomyces lividans with plasmid pIJ702.

Y Murooka, T Ishizaki, O Nimi, N Maekawa.   

Abstract

The cholesterol oxidase gene (cho) of Streptomyces sp. was cloned into Streptomyces lividans with the vector pIJ702. Deletion analysis of the recombinant plasmid showed that entire coding sequence of the cho gene was located within a 2.5-kilobase segment of the chromosomal DNA obtained from the cholesterol oxidase-producing strain. When cloned cells of S. lividans were grown in an appropriate medium, the cells produced severalfold more cholesterol oxidase extracellularly than did the producing strain.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3466572      PMCID： PMC239237          DOI： 10.1128/aem.52.6.1382-1385.1986

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  Studies on the microbiological degradation of cholesterol.

Authors:  T C STADTMAN; A CHERKES; C B ANFINSEN
Journal:  J Biol Chem       Date:  1954-02       Impact factor: 5.157

2.  Identification of the covalently bound flavin prosthetic group of cholesterol oxidase.

Authors:  W C Kenney; T P Singer; M Fukuyama; Y Miyake
Journal:  J Biol Chem       Date:  1979-06-10       Impact factor: 5.157

3.  Enzymatic determination of total serum cholesterol.

Authors:  C C Allain; L S Poon; C S Chan; W Richmond; P C Fu
Journal:  Clin Chem       Date:  1974-04       Impact factor: 8.327

4.  Purification of 3beta-hydroxysteroid oxidase of Streptomyces violascens origin by affinity chromatography on cholesterol.

Authors:  T Kamei; Y Takiguchi; H Suzuki; M Matsuzaki; S Nakamura
Journal:  Chem Pharm Bull (Tokyo)       Date:  1978-09       Impact factor: 1.645

5.  Purification and some properties of cholesterol oxidase from Schizophyllum commune with covalently bound flavin.

Authors:  M Fukuyama; Y Miyake
Journal:  J Biochem       Date:  1979-05       Impact factor: 3.387

6.  Some enzymatic properties of 3beta-hydroxysteroid oxidase produced by Streptomyces violascens.

Authors:  H Tomioka; M Kagawa; S Nakamura
Journal:  J Biochem       Date:  1976-05       Impact factor: 3.387

7.  pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.

Authors:  T Kieser; D A Hopwood; H M Wright; C J Thompson
Journal:  Mol Gen Genet       Date:  1982

8.  Cloning of antibiotic resistance and nutritional genes in streptomycetes.

Authors:  C J Thompson; J M Ward; D A Hopwood
Journal:  J Bacteriol       Date:  1982-08       Impact factor: 3.490

9.  Cloning and expression in streptomyces lividans of antibiotic resistance genes derived from Escherichia coli.

Authors:  J L Schottel; M J Bibb; S N Cohen
Journal:  J Bacteriol       Date:  1981-04       Impact factor: 3.490

10.  Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.

Authors:  E Katz; C J Thompson; D A Hopwood
Journal:  J Gen Microbiol       Date:  1983-09
View more
  18 in total

1.  Extracellular cholesterol oxidase from Rhodococcus sp.: isolation and molecular characterization.

Authors:  Hamed Lashkarian; Jamshid Raheb; Kiana Shahzamani; Hossein Shahbani; Mehdi Shamsara
Journal:  Iran Biomed J       Date:  2010 Jan-Apr

Review 2.  Traditional healthful fermented products of Japan.

Authors:  Yoshikatsu Murooka; Mitsuo Yamshita
Journal:  J Ind Microbiol Biotechnol       Date:  2008-05-07       Impact factor: 3.346

Review 3.  Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes.

Authors:  P K Tomich
Journal:  Antimicrob Agents Chemother       Date:  1988-10       Impact factor: 5.191

Review 4.  The impact of genetic engineering on the commercial production of antibiotics by Streptomyces and related bacteria.

Authors:  C R Hutchinson
Journal:  Appl Biochem Biotechnol       Date:  1987 Sep-Dec       Impact factor: 2.926

5.  Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp.

Authors:  T Ishizaki; N Hirayama; H Shinkawa; O Nimi; Y Murooka
Journal:  J Bacteriol       Date:  1989-01       Impact factor: 3.490

6.  Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.

Authors:  D R Corbin; J T Greenplate; E Y Wong; J P Purcell
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792

7.  Expression of cho and melC operons by a Streptococcus thermophilus synthetic promoter in Escherichia coli.

Authors:  D K Solaiman; G A Somkuti
Journal:  Appl Microbiol Biotechnol       Date:  1995 May-Jun       Impact factor: 4.813

8.  Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli.

Authors:  Federica Volontè; Loredano Pollegioni; Gianluca Molla; Luca Frattini; Flavia Marinelli; Luciano Piubelli
Journal:  BMC Biotechnol       Date:  2010-04-21       Impact factor: 2.563

9.  An operon containing the genes for cholesterol oxidase and a cytochrome P-450-like protein from a Streptomyces sp.

Authors:  M Horii; T Ishizaki; S Y Paik; T Manome; Y Murooka
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

10.  Secretory overproduction of Arthrobacter simplex 3-ketosteroid delta 1-dehydrogenase by Streptomyces lividans with a multi-copy shuttle vector.

Authors:  K P Choi; I Molnár; Y Murooka
Journal:  Appl Microbiol Biotechnol       Date:  1995-11       Impact factor: 4.813
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.