Literature DB >> 3322945

Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph.

D Cullen1, S A Leong, L J Wilson, D J Henner.   

Abstract

Aspergillus nidulans strain G191 was transformed to hygromycin resistance using plasmid pDH25, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the A. nidulans trpC gene. Southern hybridizations of transformants revealed multiple, integrated copies of the vector. A pleiotropic effect conferring increased hygromycin B sensitivity was found to be associated with the A. nidulans pyrG89 allele. Plasmid pDH25 features a ClaI site immediately preceding the hph start codon thus permitting convenient replacement of the trpC sequences with other eukaryotic promoters.

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Year:  1987        PMID： 3322945     DOI： 10.1016/0378-1119(87)90172-7

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  66 in total

1.  Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae.

Authors:  T Kusakabe; Y Sugimoto; Y Hirota; S Toné; Y Kawaguchi; K Koga; T Ohyama
Journal:  Mol Biol Rep       Date:  2000-03       Impact factor: 2.316

2.  The efficiency of different IRESs (internal ribosomes entry site) in monocistronic mRNAS.

Authors:  J Attal; M C Théron; S Rival; C Puissant; L M Houdebine
Journal:  Mol Biol Rep       Date:  2000-03       Impact factor: 2.316

3.  Ste12 transcription factor homologue CpST12 is down-regulated by hypovirus infection and required for virulence and female fertility of the chestnut blight fungus Cryphonectria parasitica.

Authors:  Fuyou Deng; Todd D Allen; Donald L Nuss
Journal:  Eukaryot Cell       Date:  2006-11-17

4.  Functional analysis of all nonribosomal peptide synthetases in Cochliobolus heterostrophus reveals a factor, NPS6, involved in virulence and resistance to oxidative stress.

Authors:  Bee-Na Lee; Scott Kroken; David Y T Chou; Barbara Robbertse; O C Yoder; B Gillian Turgeon
Journal:  Eukaryot Cell       Date:  2005-03

5.  Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility.

Authors:  E Mellado; G Garcia-Effron; M J Buitrago; L Alcazar-Fuoli; M Cuenca-Estrella; J L Rodriguez-Tudela
Journal:  Antimicrob Agents Chemother       Date:  2005-06       Impact factor: 5.191

6.  A split luciferase complementation assay for studying in vivo protein-protein interactions in filamentous ascomycetes.

Authors:  Hee-Kyoung Kim; Eun Ji Cho; Seong mi Jo; Bo Reum Sung; Seunghoon Lee; Sung-Hwan Yun
Journal:  Curr Genet       Date:  2012-04-25       Impact factor: 3.886

7.  Aspergillus fumigatus C-5 sterol desaturases Erg3A and Erg3B: role in sterol biosynthesis and antifungal drug susceptibility.

Authors:  Laura Alcazar-Fuoli; Emilia Mellado; Guillermo Garcia-Effron; Maria J Buitrago; Jordi F Lopez; Joan O Grimalt; J Manuel Cuenca-Estrella; Juan L Rodriguez-Tudela
Journal:  Antimicrob Agents Chemother       Date:  2006-02       Impact factor: 5.191

8.  Virulence of Aspergillus fumigatus double mutants lacking restriction and an alkaline protease in a low-dose model of invasive pulmonary aspergillosis.

Authors:  J M Smith; C M Tang; S Van Noorden; D W Holden
Journal:  Infect Immun       Date:  1994-12       Impact factor: 3.441

9.  Tagged mutations at the Tox1 locus of Cochliobolus heterostrophus by restriction enzyme-mediated integration.

Authors:  S Lu; L Lyngholm; G Yang; C Bronson; O C Yoder; B G Turgeon
Journal:  Proc Natl Acad Sci U S A       Date:  1994-12-20       Impact factor: 11.205

10.  In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids.

Authors:  W A Powell; H C Kistler
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490
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